2013 Fiscal Year Final Research Report
The relationship of caveolae on human glomerular endotherial cells with albumin permiability
| Project/Area Number |
24790862
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | カベオラ / カベオリン-1 / アルブミン / 糸球体内皮細胞 / エンドサイトーシス |
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| Research Abstract |
The purpose of this study is to elucidate whether the albumin enter into the human glomerular endotherilal cells (HRGECs) through caveolae or not.
In this experiment, we showed that the labeled albumin were co-localized with caveolin-1, which was a marker of caveolae, on HRGECs. We also showed that the uptake of albumin was dramatically decreased by the caveolae disrupting agents, such as methyl beta cholecystdextrin and nystatin. We also showed the decreasing of albumin uptake by the disrupting of caveolin-1 gean by caveolin-1 siRNA. These results indicated that albumin entered into HRGECs through caveolae, and albumin endocytosis through caveolae might become one of the new etiology of albuminuria instead of the phenestrae which is recognized as previous pathway of albumin through HRGECs.
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