2014 Fiscal Year Final Research Report
Optimization of chimeric antigen receptor-gene modified T lymphocyte generation
| Project/Area Number |
24790969
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | キメラ抗原レセプター / 細胞療法 / 遺伝子導入T細胞 / 免疫療法 / リンパ性白血病 / 悪性リンパ腫 |
|---|
| Outline of Final Research Achievements |
We aimed to optimize the method for generation of chimeric antigen receptor-gene modified T lymphocyte (CD19CAR-T). For the generation of allogeneic CD19CAR-T lymphocytes, we analyzed the method for better generation of virus-specific/ CD19CAR-gene modified T lymphocytes. We tested the possible combination of cytokine combination and concentration. As a result, the IL-2 and IL-21 combination was better than other conditions. For the optimization of autologous CD19CAR-T lymphocytes, we developed drug-inducible expression system of CD19CAR, and demonstrated that we could successfully control CD19CAR expression under drug administration.
|
| Free Research Field |
遺伝子導入T細胞療法学
|
