Analysis of the molecular function of Geminin in leukemogenesis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790976
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Hiroshima University
• Principal Investigator: OHNO Yoshinori 広島大学, 原爆放射線医科学研究所, 助教 (10548986)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 白血病幹細胞 / Geminin / Hoxa9 / Mll-Af9

Research Abstract

We demonstrated that Hoxa9 regulate the activity of hematopoietic stem cells (HSCs) through the ubiquitin-proteasom-mediated direct regulation of Geminin, indicating that Geminin acts as a central factor for regulating cellular proliferation and differentiation in HSCs. Recently, we have shown that Geminin egulates the transcriptional activity of E2F, a regulator of cell cycle, through the chromatin remodeling. These findings suggest that Geminin plays an important role in hematopoiesis and leukemogenesis

Research Products

• [Journal Article] Transcription of the Geminin gene is regulated loop (2014)
  • Author(s): Ohno Y, Saeki K, Yasunaga S, Kurogi T, Suzuki-Takedachi K, Shirai M, Mihara K, Yoshida K, Voncken JW, Ohtsubo M, Takihara Y
  • Journal Title: Mol Biol Cell Volume: 25(8) Pages: 1374-83
  • Peer Reviewed
• [Journal Article] Hoxa9 transduction induces hematopoietic stem and progenitor cell activity through direct down-regulation of geminin protein (2013)
  • Author(s): Ohno Y, Yasunaga S, Janmohamed S, Ohtsubo M, Saeki K, Kurogi T, Mihara K, Iscove NN, Takihara Y
  • Journal Title: PLoS One Volume: 8(1) Pages: e53161
  • Peer Reviewed
• [Journal Article] Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4 (2013)
  • Author(s): Yasunaga S, Ohtsubo M, K, Kurogi T, Tanaka Ishizaki H, Shi HW, Miyoshi J, Takihara Y
  • Journal Title: Mol Cell Biol Volume: 33(4) Pages: 644-60
  • Peer Reviewed
• [Presentation] Negative feed-back loop regulates E2F-mediated transcriptional activation of the Geminin gane (2013)
  • Author(s): Toshiaki Kurogi, Yoshinori Ohno, Shin'ichiro Yasunaga, Kyoko Suzuki-Takedachi, Manabu Shirai, Motoaki Ohtsubo, Yoshihiro Takihara
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20131203-06
• [Presentation] Negative feed-back loop regulates transcription of the Geminin gene, which governs HSC activity (2013)
  • Author(s): Yoshinori Ohno, Shin'ichiro Yasunaga , Toshiaki Kurogi, Kyoko Suzuki-Takedachi, Yoshie Nakashima, Motoaki Ohtsubo, Yoshihiro Takihara
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 20131011-13
• [Presentation] Scmh1 is required for maintaining hematopoietic homeostasis through the molecular network governing Geminin expression (2012)
  • Author(s): Yoshinori Ohno, Shin'ichiro Yasunaga, Keita Saeki, Motoaki Ohtsubo, Toshiaki kurogi, Yoshie Nakashima, Yoshihiro Takihara
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20121211-14
• [Presentation] Repression of Geminin plays an important role in Hoxa9-induced hematopoiesis and leukemogenesis (2012)
  • Author(s): Yoshinori Ohno, Shin'ichiro Yasunaga, Motoaki Ohtsubo, Keita Saeki, Yoshie Nakashima, Yoshihiro Takihara
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会議場
  • Year and Date: 20121019-21
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/dscb/index.html