2013 Fiscal Year Final Research Report
Analysis of human HSCs-supporting mechanisms of human bone marrow-derived mesenchymal stromal cells and development of a Human HSC expansion system
| Project/Area Number |
24790984
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 造血幹細胞 / 間葉系幹細胞 / 骨髄ニッチ |
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| Research Abstract |
I developed about 500 hybridoma clones which produce the monoclonal antibody (mAb) against human cord blood-derived 18Lin-CD34+/- fractions. In this fraction, human primitive hematopoietic stem cells (HSCs) are highly concentrated. Then, I performed screening of neutralization antibodies against the human HSC supportive activities of human bone marrow-derived mesenchymal stem cells (MSCs) using this antibody library. For this propose, these developed mAbs were added into coculture of MSC and 18Lin-CD34+/- cells. In the result, I found the antibody which inhibited (approximately 20%) the HSC supportive ability of MSCs from this antibody library. This screening is currently in progress.
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