Exploration of the new components for elastic fiber assembly

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hoshi University
• Principal Investigator: SATO Fumiaki 星薬科大学, 薬学部, 講師 (10468580)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 光老化 / 日光弾性線維症 / 弾性線維 / リジルオキシダーゼ

Research Abstract

To investigate the new components for elastic fiber assembly, we focused on the solar elastosis, of which characterization is observed the abnormal elastic fiber in adult dermis. First, we assess the mRNA expression between the dermal fibroblast from neonatal and adult after UV radiation. Because RT-qPCR showed the difference in mRNA expression which related to lysyl oxidase, we clarified the function of lysyl oxidase in elastogenesis. Lysyl oxidase is known to cross-linker between tropoelastin or collagen molecules, resulting in maturatation of elastic fibers or collagen fibers. We found that lysyl oxidase affects the deposition of tropoelastin onto microfibril fibers and localizes on the cell surface in early stage of elastic fiber assembly. Further, the release of lysyl oxidase form the cell surface was delayed in adult dermal fibroblast, resulting in abnormal assembly of elastic fiber. These data suggests that the release of lysyl oxidase is key step of elastogenesis.

Research Products

• [Journal Article] Domain 36 of tropoelastin in elastic fiber formation (2014)
  • Author(s): Nonaka R, Sato F, Wachi H
  • Journal Title: Biol.Pharm.Bull Volume: 37(4) Pages: 698-702
• [Journal Article] Trichostatin A, an HDAC class I/II inhibitor, promotes Pi-induced vascular calcification via up-regulation of the expression of alkaline phosphatase (2013)
  • Author(s): Azechi T, Kanehira D, Kobayashi T, Sudo R, Nishimura A, Sato F, Wachi H
  • Journal Title: J Atheroscler Thromb Volume: 20(6) Pages: 538-47
• [Journal Article] ATP13A2 deficiency induces a decrease in cathepsin D activity, fingerprint-like inclusion body formation, and selective degeneration of dopaminergic neurons (2013)
  • Author(s): Matsui H, Sato F, Sato S, Koike M, Taruno Y, Saiki S, Funayama M, Ito H, Taniguchi Y, Uemura N, Toyoda A, Sakaki Y, Takeda S, Uchiyama Y, Hattori N, Takahashi R
  • Journal Title: FEBS Lett Volume: 2;587(9) Pages: 1316-25
• [Journal Article] 5-aza-2′-deoxycytidine, a DNA methyltransferase inhibitor, facilitates inorganic phosphorus-induced mineralization of vascular smooth muscle cells
  • Author(s): Azechi T, Sato F, Sudo R, Wachi H
  • Journal Title: J Atheroscler Thromb Volume: (in press)
• [Presentation] (2013)
  • Author(s): 里 史明
  • Organizer: 第45回日本結合組織学会学術大会/第60回マトリックス研究会大会合同学術大会
  • Place of Presentation: 和歌山
  • Year and Date: 20130600
• [Presentation] (2013)
  • Author(s): Sato F
  • Organizer: Gordon Research Confecrence on Elastin, Elastic fibers & Microfibrils
  • Place of Presentation: USA
  • Year and Date: 20130000
• [Presentation] (2012)
  • Author(s): 西村 章子, 里 史明, 輪千 浩史
  • Organizer: 第10回日本エラスチン研究会
  • Place of Presentation: 東京
  • Year and Date: 20121200
• [Presentation] (2012)
  • Author(s): 畦地 拓哉, 磯川 桂太郎, 山崎 洋介, 湯口眞紀, 里 史明, 輪千 浩史
  • Organizer: 第10回日本エラスチン研究会
  • Place of Presentation: 東京
  • Year and Date: 20121200
• [Presentation] (2012)
  • Author(s): 須藤 涼, 里 史明, 輪千 浩史
  • Organizer: 第10回日本エラスチン研究会
  • Place of Presentation: 東京
  • Year and Date: 20121200