Development of in-vivo imaging method for immune cell tracking in mouse brain by using magnetic resonance imaging

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791302
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: MORI Yuki 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (10559355)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 磁気共鳴画像法 / 免疫細胞動態 / 細胞追跡 / 神経免疫学

Research Abstract

We established highly sensitive in-vivo imaging techniques for sequential monitoring of cell migration in the mouse brain at the single-cell level by using 11.7 T high-field magnetic resonance imaging (MRI) scanner. MRI with intravenous administration of superparamagnetic particles of iron oxide (SPIO) can be used to successfully monitor the transmigration of peripheral phagocytes into healthy and inflamed brains, as intracellular absorbed-SPIO can pass through the blood-brain barrier. We successfully detected single cells in live animal brains by achieving images of high spatio-temporal resolution as a result of high SNR. In addition to the high magnetic field, improvements in MR techniques; such as the custom-made RF coil, optimization of MR parameters and optimization of contrast agents, were significant factors for this achievement.

Research Products

• [Journal Article] 磁性粒子を用いた抹消組織マクロファージの脳内浸潤の経時的観察 (2013)
  • Author(s): 森 勇樹, 他
  • Journal Title: 日本磁気共鳴医学会雑誌 Volume: 34巻 Pages: 14-17
  • Peer Reviewed
• [Journal Article] 磁気共鳴法を用いたダイナミックな免疫現象の可視化を目指して (2012)
  • Author(s): 森 勇樹, 朱 大松, 吉岡 芳親
  • Journal Title: JSMI Report Volume: Vol.5 Pages: 9-14
  • Peer Reviewed
• [Journal Article] Early pathological alterations of lower lumbar cords detected by ultrahigh-field MRI in a mouse multiple sclerosis model
  • Author(s): Mori Y, et al
  • Journal Title: International Immunology Volume: Vol.26, No. 2 Pages: 93-101
  • Peer Reviewed
• [Presentation] Sequential and time-lapse MRI monitoring of peripheral macrophage recruitment and migration in mouse brain (2014)
  • Author(s): Mori Y
  • Organizer: 23rd annual meeting of international society of magnetic resonance in medicine
  • Place of Presentation: Milan, Italy
  • Year and Date: 20140510-16
• [Presentation] MRイメージングを用いた経時的免疫細胞動態追跡 (2014)
  • Author(s): 森 勇樹
  • Organizer: 第2回熊本大学イメージングセミナー
  • Place of Presentation: 熊本
  • Year and Date: 2014-04-25
  • Invited
• [Presentation] MRイメージングを用いた経時的脳内免疫細胞動態追跡 (2014)
  • Author(s): 森 勇樹
  • Organizer: 第2回臨床免疫核医学研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-01-20
  • Invited
• [Presentation] In vivo MRI monitoring of immune cell migration in mouse brain by using superparamagnetic nanoparticles (2013)
  • Author(s): Mori Y
  • Organizer: International symposium on nanomedicine
  • Place of Presentation: 福岡
  • Year and Date: 20131107-09
  • Invited
• [Presentation] 高感度MRI装置を用いた多発性硬化症モデルマウス腰髄における急性期病態変化の評価 (2013)
  • Author(s): 森 勇樹
  • Organizer: 第41回日本磁気共鳴医学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20130919-21
• [Presentation] 新規酸化鉄増影剤とリゾビストのマウス体内分布の比較 (2013)
  • Author(s): 陳 挺, 森 勇樹, 他
  • Organizer: 第41回日本磁気共鳴医学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20130919-21
• [Presentation] 磁性粒子を用いた抹消組織マクロファージの脳内浸潤の経時的観察 (2013)
  • Author(s): 森 勇樹
  • Organizer: 第41回日本磁気共鳴医学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20130919-21
• [Presentation] In vivo monitoring of immune cell migration in mouse brain by using high-field MRI (2013)
  • Author(s): Mori Y
  • Organizer: 13th European school of neuroimmunology
  • Place of Presentation: Porto, Portugal
  • Year and Date: 20130703-06
• [Presentation] Temporal changes in lower-lumbar spinal cord in EAE mouse (2013)
  • Author(s): Mori Y
  • Organizer: 22nd annual meeting of international society of magnetic resonance in medicine
  • Place of Presentation: Salt Lake City, USA
  • Year and Date: 20130420-26
• [Presentation] In vivo visualization of immune cells in the central nervous system (2012)
  • Author(s): Yoshioka Y, Mori Y
  • Organizer: Towards comprehensive understanding of immune dynamism (TCUID 2012)
  • Place of Presentation: 大阪
  • Year and Date: 20121029-31
• [Presentation] 正常及び病態モデルマウスにおける脳内免疫細胞動態の描出 (2012)
  • Author(s): 森 勇樹
  • Organizer: 第40回日本磁気共鳴医学会大会
  • Place of Presentation: 京都
  • Year and Date: 20120906-08
• [Presentation] Non-invasive visualization of immunological responses in mice with high field MRI at 11.7 T (2012)
  • Author(s): Mori Y
  • Organizer: 8th international forum on multimedia and image processing, World automation congress
  • Place of Presentation: Puerto Vallarta, Mexico
  • Year and Date: 20120624-28
• [Presentation] Visualization of immune cell dynamics in mouse brain with MRI (2012)
  • Author(s): Mori Y
  • Organizer: 21st annual meeting of international society of magnetic resonance in medicine
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20120505-11
• [Remarks] 第41回日本磁気共鳴医学会大会学術奨励賞(大会長賞)受賞(ポスタータイトル:磁気性粒子を用いた抹消組織マクロファージの脳内浸潤の経時的観察), 2013年9月20日
• [Remarks] 日本磁気共鳴医学会学会賞 バイエル学術奨励賞国際飛躍賞 受賞(受賞対象発表:Temporal Changes in Lower-Lunbar Spinal Cord in EAE Mouse(多発生硬化症モデルマウス下位腰髄における急性期病態変化の評価)) 2013年12月18日
• [Remarks]
  • URL: http://biofunc.ifrec.osaka-u.ac.jp