MicroRNAs and their potential targets associated with characteristics of estrogen receptor-positive breast cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Nagoya City University
• Principal Investigator: ENDO Yumi 名古屋市立大学, 医学(系)研究科(研究院), 助教 (20566228)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: マイクロRNA

Research Abstract

Recent analyses have identified heterogeneity in ER-positive breast cancer. Subtypes called luminal A and luminal B have been identified, and the tumor characteristics are different in these subtypes. In this study, expression profiles of microRNAs and mRNAs in ER-positive breast cancer tissue were compared between luminal A tumors and luminal B tumors by miRNA and mRNA microarrays.
Unsupervised hierarchical clustering analyses revealed distinct expression patterns of miRNAs and mRNAs. We identified a down-regulation of miR-1290 in luminal A tumors. We picked up 11 genes that were potential target genes of the selected miRNAs. Protein expression patterns of the selected target genes were analyzed in ER-positive breast cancer samples by immunohistochemistry: miR-1290 and its 4 putative targets, BCL2, FOXA1, MAPT and NAT1 were identified. Transfection experiments revealed that introduction of miR-1290 into ER-positive breast cancer cells decreased mRNA and protein expression of NAT1.

Research Products

• [Journal Article] miR-1290 and its potential targets are associated with characteristics of estrogen receptor α-positive breast cancer (2013)
  • Author(s): Endo Y, Toyama T, Takahashi S, Yoshimoto N, Iwasa M, Asano T, Fujii Y, Yamashita H
  • Journal Title: Endocr Relat Cancer Volume: 18;20(1) Pages: 91-102
  • DOI: 10.1530/ERC-12-0207
  • Peer Reviewed
• [Presentation] Arylamine N-acetyltransferase 1 (NAT1)はmiR-1290 の標的遺伝子であり, 乳癌の予後因子である (2014)
  • Author(s): 遠藤友美, 山下啓子, 高橋智, 佐藤慎哉, 吉本信保, 浅野倫子, 波戸ゆかり, 董宇, 藤井義敬, 遠山竜也
  • Organizer: 第114回 日本外科学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 20140403-05
• [Presentation] MiR-1290 and its potential targets are associated with characteristics of estrogen receptor α-positive breast cancer (2013)
  • Author(s): Yumi Endo, Tatsuya Toyama, Satoru Takahashi, Nobuyasu Yoshimoto, Mai Iwasa, Tomoko Asano, Yoshitaka Fujii and Hiroko Yamashita
  • Organizer: ASCO
  • Place of Presentation: Chicago, USA
  • Year and Date: 20130531-0604
• [Presentation] MicroRNAs and their potential targets associated with characteristics of estrogen receptor-positive breast cancer (2013)
  • Author(s): Yumi Endo, Tatsuya Toyama, Satoru Takahashi, Nobuyasu Yoshimoto, Mai Iwasa, Tomoko Asano, Yoshitaka Fujii and Hiroko Yamashita
  • Organizer: St. Gallen International Breast Cancer Conference
  • Place of Presentation: St. Gallen, Switzerland
  • Year and Date: 20130313-16
• [Presentation] MicroRNAs and their potential targets associated with characteristics of estrogen receptor-positive breast cancer (2012)
  • Author(s): Yumi Endo, Tatsuya Toyama, Satoru Takahashi, Nobuyasu Yoshimoto, Mai Iwasa, Tomoko Asano, Yoshitaka Fujii and Hiroko Yamashita
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] エストロゲンレセプター陽性乳癌の生物学的特性に関与するmicroRNA とその標的遺伝子の解析 (2012)
  • Author(s): 遠藤友美, 高橋智, 遠山竜也, 吉本信保, 岩佐麻衣, 浅野倫子, 山下啓子
  • Organizer: 第20回日本乳癌学会学術総会
  • Place of Presentation: 熊本
  • Year and Date: 20120628-30
• [Presentation] microRNAs and their target proteins associated with characteristics of estrogen receptor-positive breast cancer (2011)
  • Author(s): Yumi Endo, Satoru Takahashi, Tatsuya Toyama, Nobuyasu Yoshimoto, Mai Iwasa, Yoshitaka Fujii, Hiroko Yamashita
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04