2014 Fiscal Year Final Research Report
Elucidation of the role of cytoskeletal proteins in circulating tumor cells of pancreatic cancer
| Project/Area Number |
24791451
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Toyo University (2014)
Hokkaido University (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | LC/MS / 膵癌 |
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| Outline of Final Research Achievements |
It was confirmed that it is possible to keep then in advance labeled CTCs generated by the orthotopically transplanted fluorescence-labeled human pancreatic cancer cell lines. Therefore, in this experimental system can be expected to contribute significantly to the detailed cell analysis of CTCs in the future. Cytoskeletal proteins such as nestin, E-cadherin and vimentin have enabled high-sensitivity detection by LC / MS. In the future, it is necessary to consider whether can be detected in the crude protein of cultured cells. Upon absolute quantification method by LC / MS is established, in order to evaluate the expression pattern of EMT-related proteins in pancreatic cancer cell, which is the next target, and is expected to continue to compare with the expression pattern of cancer cells other than pancreatic cancer.
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| Free Research Field |
医歯薬学
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