The effectiveness of erythropoietin for discogenic low back pain

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791523
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: INOUE Gen 北里大学, 医学部, 講師 (80594209)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: シュワン細胞 / 椎間板細胞 / 疼痛

Research Abstract

The effectiveness of erythropoetin for rats discogenic low back pain was evaluated. The cells from nucleus pulpous were isolated and cultured, and supernatant of medium was obtained. This supernatant, which includes many kinds of cytokines and growth factors stimulates primary Schwann cells and the expression of MAP kinase were evaluated. Pretreatment with erythropoietin suppressed MAP kinase expression significantly. Also, in vivo, erythropoietin suppressed MAP kinase expression in dorsal root ganglion and glial activation in dorsal horn in spinal cord. Erythropoietin could be one of the effective treatment for discogenic low back pain.

Research Products

• [Journal Article] Evaluation of pain behavior and calcitonin gene-related peptide immunoreactive sensory nerve fibers in the spinal dorsal horn after sciatic nerve compression and application of nucleus pulposus in rats (2014)
  • Author(s): Kimura S, Sakuma Y, Suzuki M, Orita S, Yamauchi K, Inoue G, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Kubota G, Oikawa Y, Inage K, Sainoh T, Sato J, Nakamura J, Toyone T, Takahashi K, Ohtori S
  • Journal Title: Spine Volume: 39巻 Pages: 455-462
  • DOI: 10.1097/BRS.0000000000000180
  • Peer Reviewed
• [Journal Article] Upregulation of NaV1.7 in dorsal root ganglia after intervertebral disc injury in rats (2014)
  • Author(s): Sadamasu A, Sakuma Y, Suzuki M, Orita S, Yamauchi K, Inoue G, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Kubota G, Oikawa Y, Inage K, Sainoh T, Sato J, Nakamura J, Toyone T, Takahashi K, Ohtori S
  • Journal Title: Spine Volume: 39巻 Pages: E421-426
  • DOI: 10.1097/BRS.0000000000000229
  • Peer Reviewed
• [Journal Article] Dichotomizing sensory nerve fibers innervating both the lumbar vertebral body and the area surrounding the iliac crest : a possible mechanism of referred lateral back pain from lumbar vertebral body (2013)
  • Author(s): Fujii T, Sakuma Y, Orita S, Inoue G, Ochiai N, Kuniyoshi K, Aoki Y, Ishikawa T, Miyagi M, Kamoda H, Suzuki M, Kubota G, Inage K, Sainoh T, Sato J, Yamauchi K, Toyone T, Nakamura J, Kishida S, Takahashi K, Koshi T, Ohtori S
  • Journal Title: Spine Volume: 38巻 Pages: E1571-1574
  • DOI: 10.1097/BRS.0b013e3182a879cd
  • Peer Reviewed
• [Journal Article] Assessment of pain behavior in a rat model of intervertebral disc injury using the CatWalk gait analysis system (2013)
  • Author(s): Miyagi M, Ishikawa T, Kamoda H, Suzuki M, Sakuma Y, Orita S, Oikawa Y, Aoki Y, Toyone T, Takahashi K, Inoue G, Ohtori S
  • Journal Title: Spine Volume: 38巻 Pages: 1459-1465
  • DOI: 10.1097/BRS.0b013e318299536a
  • Peer Reviewed
• [Journal Article] Comparison of CatWalk analysis and von Frey testing for pain assessment in a rat model of nerve crush plus inflammation (2013)
  • Author(s): Sakuma T, Kamoda H, Miyagi M, Ishikawa T, Arai G, Eguchi Y, Suzuki M, Oikawa Y, Sakuma Y, Kubota G, Inage K, Saino T, Orita S, Yamauchi K, Inoue G, Takahashi K, Ohtori S
  • Journal Title: Spine Volume: 38巻 Pages: E919-924
  • DOI: 10.1097/BRS.0b013e318297bfb6
  • Peer Reviewed
• [Journal Article] In-vivo transfection of the proopiomelanocortin gene, precursor of endogenous endorphin, by use of radial shock waves alleviates neuropathic pain (2013)
  • Author(s): Ishikawa T, Miyagi M, Yamashita M, Kamoda H, Eguchi Y, Arai G, Suzuki M, Sakuma Y, Oikawa Y, Orita S, Inoue G, Ozawa T, Aoki Y, Toyone T, Takahashi K, Yamaguchi A, Ohtori S
  • Journal Title: J Orthop Sci Volume: 18巻 Pages: 636-645
  • DOI: 10.1007/s00776-013-0397-y
  • Peer Reviewed
• [Presentation] 慢性疼痛における塩基性線維芽細胞増殖因子の役割-痛みへの関与と治療への可能性 (2013)
  • Author(s): 井上玄, 内田健太郎, 上野正喜, 齋藤亘, 藤巻寿子, 中澤俊之, 井村貴之, 高平尚伸, 高相晶士
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉市
  • Year and Date: 2013-10-18
• [Presentation] Quantitative measurement of rats myofascial low back pain using CatWalk gait analysis system (2013)
  • Author(s): Inoue G, Ohtori S, Miyagi M, Ishikawa T, Kamoda H, Eguchi Y, Suzuki M, Sakuma Y, Oikawa Y, Takaso M, Takahashi K
  • Organizer: The 14th EFFORT Congress
  • Place of Presentation: イスタンブール(トルコ)
  • Year and Date: 2013-06-05