The development of a specific anti-OPN antibody which contributes to to the remarkable inhibition of early stage of renal crystal formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791663
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: SHUZO Hamamoto 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (80551267)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腎結石 / オステオポンチン

Outline of Final Research Achievements

Osteopontin plays a crucial role in the formation of renal calcium crystals, which are primarily induced by renal tubular cell injury, especially mitochondrial damage. We have previously shown that the impaired RGD sequence of osteopontin inhibits renal crystal formation by using OPN-transgenic mice and OPN-knockout mice. Here, we investigated the effects of an anti-murine osteopontin antibody (35B6-Ab) that specifically reacts with the 162SLAYGLR168 sequence, which is exposed by thrombin cleavage and is located adjacent to the RGD sequence, on renal crystal formation. We conclude that thrombin-cleaved osteopontin plays an important role in formation of renal calcium crystals and that 35B6-Ab contributes to the remarkable inhibition of early-stage renal crystal formation by preventing renal tubular cell injury and crystal-cell attachment.

Free Research Field

医歯薬学