2013 Fiscal Year Final Research Report
Epigenetics regulation of steroid syntesis related gene promoter regions in granulosa cells undergoing luteinization during ovulation in rats
| Project/Area Number |
24791705
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
LEE Lifa 山口大学, 医学部附属病院, 助教 (90610668)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | エピジェネティクス / プロゲステロン合成 |
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| Research Abstract |
The ovulatory LH surge rapidly alters the expression of steroidogenesis-related genes in granulosa cells. Steroidogenic acute regulatory protein (StAR) is rapidly induced and aromatase (Cyp19a1) is rapidly suppressed after the LH surge. The rapid shift of estrogen synthesis to progesterone synthesis after the LH surge is crucial for ovulation and the following luteinization. We revealed changes of histone modification status and chromatin structure in the StAR and Cyp19s1 promoter region in addition to DNA hypomethylation status of the promoter are closely associated with the rapid change of StAR and Cyp19a1 mRNA expression in granulosa cells undergoing luteinization during ovulation.
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