Autophagy regulates galectin-4 expression in the rat trophoblast cell line and affects its differentaiton

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791726
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa Medical University
• Principal Investigator: ARIKAWA Tomohiro 金沢医科大学, 一般教育機構, 講師 (70452670)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: Galectin-４ / Autophagy / MMP / Ｉnvasive trophoblast / Ｐｌａｃｅｎｔａ

Research Abstract

This study aimed to investigate the expression of Gal4 in placentation. We utilized Rcho-1 cells derived from rat placenta and this cell line has been well established as a model system for trophoblast differentiation. The expression of Gal4 was revealed to be lower in differentiated Rcho-1 cells than that in proliferative cells. Of interest, overexpression of Gal4 during Rcho-1 differentiation resulted in moderate reduction of both cell mobility and cell enlargement. Notably, immunohistochemical analysis showed that Gal4 is preferentially located in the maternal-fetal zone of rat placenta, indicating that Gal4 would negatively regulate differentiation of Rcho-1 cells and/or limit their invasive property.

Research Products

• [Journal Article] T cells control innate hyperinflammation in LPS endotoxemia by downregulating macrophage TNF production via IRAK-1-mediated IL-10 expression (2014)
  • Author(s): Inoue M, Arikawa T, Chen YH, Moriwaki Y, Price M, Brown M, Perfect JR, Shinohara ML
  • Journal Title: Proceedings of National Academy of Sciences of the United States of America Volume: (in press)
  • Peer Reviewed
• [Journal Article] Significance of sugar chain recognition by galectins and its involvement in disease-associated glycosylation (2014)
  • Author(s): Arikawa T, Simamura E, Shimada H, Nakamura T, Hatta T, and Shoji H
  • Journal Title: Congenital Anomalies Volume: (in press)
  • Peer Reviewed
• [Journal Article] Inhibition of influenza A infection by galectin-9 (2013)
  • Author(s): Hattori T, Arikawa T, Fujioka Y, Maruyama J, Nakayama Y, Ohba Y, Niki T, Miyazaki T, Hirashima M, and Kida H
  • Journal Title: Japanese Journal of Veterinary Research Volume: 61(1&2) Pages: 5-18
  • Peer Reviewed
• [Journal Article] 「ガレクチンによる糖鎖認識と免疫における意義」(An emerging role of sugar chain recognition by Galectins in immunity) (2013)
  • Author(s): 有川智博, 東海林博樹, 平島光臣
  • Journal Title: 臨床化学 Volume: 42 (1) Pages: 36-43
• [Journal Article] Galectin-9 signaling prolongs survival in murine lung-cancer by inducing macrophages to differentiate into plasmacytoid dendritic cell-like macrophages (2012)
  • Author(s): Kadowaki T, Arikawa T, Shinonaga R, Oomizu S, Inagawa H, Soma G, Niki T, Hirashima M
  • Journal Title: Clinical Immunology Volume: 142(3) Pages: 296-307
  • Peer Reviewed
• [Journal Article] Galectin-9 suppresses Th17 cell development in an IL-2-dependent but Tim-3-independent manner (2012)
  • Author(s): Oomizu S, Arikawa T, Niki T, Kadowaki T, Ueno M, Nishi N, Yamauchi A, and Hirashima M
  • Journal Title: Cinical Immunology Volume: 143(1) Pages: 51-8
  • Peer Reviewed
• [Journal Article] Expression pattern of Galectin 4 in rat placentation (2012)
  • Author(s): Arikawa T, Simamura E, Shimada H, Nishi N, Tatsuno T, Ishigaki Y, Tomosugi naohisa, Yamahiro C, Hata T, Takegami T, Nakamura T, Mogami H, Yamaguchi K, Otani H, Hatta T, and Shoji H
  • Journal Title: Placenta (Short Communication) Volume: 33(10) Pages: 885-7
  • Peer Reviewed
• [Journal Article] Cell surface galectin-9 expressing Th cells regulate Th17 and Foxp3+ Treg development by secreting galectin-9 (2012)
  • Author(s): Oomizu S, Arikawa T, Niki T, Kadowaki T, Ueno M, Nishi N, Yamauchi A, and Hirashima M.
  • Journal Title: PLoS ONE Volume: 7(11) Pages: e48574
  • Peer Reviewed
• [Presentation] Role of autophagy in rat trophoblast differentiation (2013)
  • Author(s): 有川智博, 森尾佳苗, 島村英理子, 島田ひろき, 八田稔久, 東海林博樹
  • Organizer: 大36回 日本分子生物学会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 20131203-06
• [Presentation] マウスγ ヘルペスウイルスはIL-27 依存的に肺炎症を惹起する (2013)
  • Author(s): 渡部明子, 伊藤龍生, 有川智博, 榊原修平, 菊谷仁, 安居輝人, 義江修
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 20131110-12
• [Presentation] Galectin-9 は細胞およびマウスにおけるインフルエンザA ウイルスの感染を抑制する.服部 ともえ (2012)
  • Author(s): 丸山容一朗, 中山洋佑, 大場雄介, 仁木敏郎, 有川智博, 宮崎忠昭, 平島光臣, 喜田宏
  • Organizer: 第60回 日本ウイルス学会
  • Place of Presentation: 大阪(グランキューブ大阪)
  • Year and Date: 20121113-15
• [Presentation] Analysis of the Galectin 4 expression during trophoblast differentiation in rat placentation (2012)
  • Author(s): Arikawa T, Simamura E, Shimada H, Hatta T, Shoji H
  • Organizer: 国際胎盤学会
  • Place of Presentation: 広島(広島国際会議場)
  • Year and Date: 20120918-21
• [Presentation] Regulated expression of Galectin-4 in rat placentation (2012)
  • Author(s): Arikawa T, Simamura E, Shimada H, Otani H, Hatta T, and Shoji H
  • Organizer: 先天異常学会
  • Place of Presentation: 東京(東京女子医科大学)
  • Year and Date: 20120706-08