Development of vaccine eye drop against Staphylococcus aureus

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791858
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Ehime University
• Principal Investigator: SUZUKI Takashi 愛媛大学, 医学部附属病院, 講師 (70398048)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 黄色ブドウ球菌 / タイコ酸 / MRSA / ワクチン / 遺伝子 / 病原因子

Research Abstract

ST764 were the most frequently identified sequence types in MRSA strains from ocular site. ST764 has a mutation in psm-mec which regulates virulence factors, and increase virulence.
Wall teichoic acids (WTAs) which are major polyanionic polymer components the cell wall of Staphylococcus aureus, plays a critical role in keratitis, and WTA appears to be a viable target for treating S. aureus infection. We developed screening methods for WTA inhibitors, and found some candidates.

Research Products

• [Journal Article] Pseudomonas aeruginosa MucD protease mediates keratitis by inhibiting neutrophil recruitment and promoting bacterial survival (2014)
  • Author(s): Mochizuki Y , Suzuki T, Oka N, Zhang Y , Hayashi Y , Hayashi N, Gotoh N, Ohashi Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55 Pages: 240-6
  • Peer Reviewed
• [Journal Article] Genotypic characterization of Staphylococcus aureus isolates from cases of keratitis and healthy conjunctival sacs (2014)
  • Author(s): Hayashi S, Suzuki T, Y amaguchi S, Inoue T , Ohashi Y
  • Journal Title: Cornea Volume: 33 Pages: 72-6
  • Peer Reviewed
• [Journal Article] Ocular Penetration and Efficacy of Levofloxacin Using Different Drug-Delivery T echniques for the Prevention of Endophthalmitis in Rabbit Eyes with Posterior Capsule Rupture (2014)
  • Author(s): Uda T , Suzuki T, Mitani A, Tasaka Y , Kawasaki S, Mito T , Ohashi Y
  • Journal Title: J Ocul Pharmacol Ther Volume: 30 Pages: 333-339
  • Peer Reviewed
• [Journal Article] Moxifloxacin as Postoperative Prophylaxis for Enterococcus faecalis-Induced Endophthalmitis After Cataract Surgery in Aphakic Rabbits (2013)
  • Author(s): Tasaka Y , Suzuki T , Kawasaki S, Uda T , Mito T , Uno T , Ohashi Y
  • Journal Title: J Ocul Pharmacol Ther Volume: 29 Pages: 403-9
  • Peer Reviewed
• [Journal Article] Combination effect of antibiotics against bacteria isolated from keratitis using fractional inhibitory concentration index (2013)
  • Author(s): Suzuki T, Ohashi Y
  • Journal Title: Cornea Volume: 32 Pages: e156-60
  • Peer Reviewed
• [Journal Article] Prospective clinical evaluation of 1.5% levofloxacin ophthalmic solution in ophthalmic perioperative disinfection (2013)
  • Author(s): Suzuki T , Tanaka H, T oriyama K, Okamoto S, Urabe K, Hashida M, Shinkai Y , Ohashi Y
  • Journal Title: J Ocul Pharmacol Ther Volume: 29 Pages: 887-92
  • Peer Reviewed
• [Journal Article] New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes (2013)
  • Author(s): Tasaka Y , Minami N, Suzuki T, Kawasaki S, Zheng X, Shiraishi A, Uno T , Miyake K, Ohashi Y
  • Journal Title: BMC Ophthalmol Volume: 13 Pages: 47
  • Peer Reviewed
• [Journal Article] Serrated Margins in Pseudomonas aeruginosa Keratitis (2013)
  • Author(s): Ishikawa E, Suzuki T, Y amaguchi S, Inoue T , Uno T , Ohashi Y
  • Journal Title: Case Rep Ophthalmol Volume: 4 Pages: 12-5
  • Peer Reviewed
• [Journal Article] Coincidence of Varicella-Zoster Virus Anterior Uveitis in a Patient with Chandler's Syndrome (2013)
  • Author(s): Joko T , Suzuki T, Inoue T , Kikuchi M, Hara Y , Shiraishi A, Ohashi Y
  • Journal Title: Case Rep Ophthalmol Volume: 4 Pages: 274-8
  • Peer Reviewed
• [Journal Article] Conjunctivitis caused by Neisseria gonorrhoeae isolates with reduced cephalosporin susceptibility and multidrug resistance (2013)
  • Author(s): Suzuki T, Kitagawa Y , Maruyama Y , Y amaguchi S, Sakane Y , Miyamoto H, Ohashi Y
  • Journal Title: J Clin Microbiol Volume: 51 Pages: 4246-8
  • Peer Reviewed
• [Journal Article] Development of a Peptide Antagonist against fsr Quorum Sensing of Enterococcus faecalis (2013)
  • Author(s): Nakayama J, Y okohata R, Sato M, Suzuki T, Matsufuji T , Nishiguchi K, Kawai T , Y amanaka Y , Nagata K, Tanokura M, Sonomoto K
  • Journal Title: ACS Chem Biol Volume: 8 Pages: 804-11
  • Peer Reviewed
• [Journal Article] Spontaneous Bleb Formation in a Presumed Pellucid Marginal Corneal Degeneration With Acute Hydrops (2013)
  • Author(s): Toriyama K, Inoue T , Suzuki T, Higashiura R, Maeda N, Ohashi Y
  • Journal Title: Cornea Volume: 32 Pages: 839-41
  • Peer Reviewed
• [Journal Article] Gilmore MS Wall teichoic acid protects Staphylococcus aureus from inhibition by Congo red and other dyes (2012)
  • Author(s): Suzuki T, Campbell J, Kim Y , Swoboda JG, Mylonakis E, Walker S
  • Journal Title: J Antimicrob Chemother Volume: 67 Pages: 2143-51
  • Peer Reviewed
• [Journal Article] Effect of photodynamic therapy with methylene blue on Acanthamoeba in vitro (2012)
  • Author(s): Mito T , Suzuki T, Kobayashi T , Zheng X, Hayashi Y , Shiraishi A, Ohashi Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 53 Pages: 6305-13
  • Peer Reviewed
• [Journal Article] Use of 5-cyano-2, 3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents (2012)
  • Author(s): Kobayashi T , Mito T , Watanabe N, Suzuki T, Shiraishi A, Ohashi Y
  • Journal Title: J Clin Microbiol Volume: 50 Pages: 1606-12
  • Peer Reviewed
• [Journal Article] Cytomegalovirus retinitis after multiple ocular surgeries in an immunocompetent patient (2012)
  • Author(s): Toriyama K, Suzuki T, Hara Y , Ohashi Y
  • Journal Title: Case Report Ophthalmol Volume: 3 Pages: 356-9
  • Peer Reviewed
• [Journal Article] Real-time polymerase chain reaction for diagnosing acyclovir-resistant herpetic keratitis based on changes in viral DNA copy number before and after treatment (2012)
  • Author(s): Inoue T , Kawashima R, Suzuki T, Ohashi Y
  • Journal Title: Arch Ophthalmol Volume: 130 Pages: 1462-4
  • Peer Reviewed
• [Journal Article] Genotypic analysis of Pseudomonas aeruginosa isolated from ocular infection
  • Author(s): Yamaguchi S, Suzuki T, Kobayashi T , Oka N, Ishikawa E, Shinomiya H, Ohashi Y
  • Journal Title: J Infect Chemother Volume: (In press)
  • Peer Reviewed
• [Presentation] Developing new treatments for infectious keratitis (2013)
  • Author(s): T . Suzuki
  • Organizer: APAO-AIOS 2013
  • Place of Presentation: Hyderabad India
  • Year and Date: 20130117-20
• [Presentation] The evaluation of antibiotics in the prophylaxis or treatment of endophthalmitis using animal model (2012)
  • Author(s): T . Suzuki
  • Organizer: Korean Ophthalmological Society 2012
  • Place of Presentation: Seoul Korea
  • Year and Date: 20121104-05
• [Presentation] Interaction between ophthalmic viscosurgical device and intraocular lens (2012)
  • Author(s): M. Arisa, T .Suzuki, Y . Hiramatsu, Y .Tasaka, T .Uda, S. Kawasaki, Y .Ohashi
  • Organizer: ESCRS 2012
  • Place of Presentation: Milan Italia
  • Year and Date: 20120908-12
• [Presentation] Visualization of the fluid dynamics under the intraocular lens in irrigation and aspiration (2012)
  • Author(s): T .Uda, M.Arisa, Y .Tasaka, S.Kawasaki, T .Suzuki, Y .Ohashi
  • Organizer: ESCRS 2012
  • Place of Presentation: Milan Italia
  • Year and Date: 20120908-12
• [Presentation] Clinical evaluation of 1.5% Levofloxacin (L VFX) ophthalmic solution in ophthalmic perioperative disinfection (2012)
  • Author(s): T . Suzuki, K.T oriyama, S. Okamoto, H.Tanaka, K.Urabe, M.Hashida , Y .Shinkai, Y .Ohashi
  • Organizer: ESCRS 2012
  • Place of Presentation: Milan Italia
  • Year and Date: 20120908-12
• [Presentation] Genotypic Characterization of Staphylococcus aureus isolates from Eyes with Keratitis (2012)
  • Author(s): T . Suzuki, S Hayashi. Y Ohashi
  • Organizer: ARVO 2012
  • Place of Presentation: FortLauderdale U.S
  • Year and Date: 20120506-09
• [Presentation] Effect of photodynamic therapy with methylene blue against Acanthamoeba In Vitro (2012)
  • Author(s): T . Mito, T .Suzuki, T . Kobayashi, A. Shiraishi, Y . Ohashi
  • Organizer: ARVO 2012
  • Place of Presentation: FortLauderdale U.S
  • Year and Date: 20120506-09
• [Presentation] Performance of conventional and new methods for the detection of pathogens in ocular infections. Frontiers in Ocular infection (2012)
  • Author(s): T . Suzuki
  • Organizer: Lunch symposium at APAO 2012
  • Place of Presentation: Busan Korea
  • Year and Date: 2012-04-14