2013 Fiscal Year Final Research Report
The elucidation of functional regulation of osteoclasts and improvements of bone metabolism by estrogen and estrogen responsive genes
| Project/Area Number |
24791963
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
HIYAMA Shinji 広島大学, 医歯薬保健学研究院(歯), 助教 (60314754)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 口腔解剖学 / 骨代謝 / エストロゲン / 破骨細胞 / エストロゲン応答遺伝子 |
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| Research Abstract |
Estrogen inhibited bone resorption, but did not induce apoptosis of osteoclasts that derived from bone marrow cells of estrogen-treated male quails. Further, the morphology of osteoclasts and the expression of ATPase in these cells were affected by estrogen administration. As a result of DNA microarray, a lot of genes that were significantly up- or down-regulated by estrogen administration were identified in these osteoclasts. Osteoclastogenesis from bone marrow cells were suppressed by knock-down of some of genes with/without estrogen. Taken together with these results, estrogen has no effects on the apoptosis of medullary bone osteoclasts, but rather suppress bone resorption. Additionally, it is suggested that many genes that indicated significant regulation of expression are related to the inhibition of apoptosis as estrogen responsive genes.
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