2013 Fiscal Year Final Research Report
Transposon mutagenesis of biofilm-forming Rothia mucilaginosa
| Project/Area Number |
24791975
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオフィルム / Rothia mucilaginosa / 遺伝子 |
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| Research Abstract |
Clinical isolate (strain DY-18) of Rothia mucilaginosa from persistent apical periodontitis produce a large amount of exopolysaccharide (EPS). In this study, to identify the genes involved in biofilm formation in R. mucilaginosa, we established EPS non-producing mutants from strain DY-18 by random mutagenesis. The viscosity of culture medium in these mutants were lower than the parent strain culture. Scanning electron microscopic observations on cell surface revealed that these mutans lacked or had significantly decreased meshwork-like structures, which is typical for biofilm-forming bacteria. We compared genes expression profiles of strain DY-18 between in static culture producing large amount of EPS, and shake culture, EPS non-producing condition, by microarray. The results in this study indicate that expression of stress response genes in the static culture is higher than that in the shaking growth condition and suggest that these genes might involve in biofilm formation.
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[Journal Article] Biofilm-forming capacity on clinically isolated Streptococcus constellatus from an odontogenic subperiosteal abscess lesion2013
Author(s)
Yamanaka T, Furukawa T, Yamane K, Nambu T, Mashimo C, Maruyama H, Inoue J, Kamei M, Yasuoka H, Horiike S, Leung KP, Fukushima H
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Journal Title
Journal of Bacteriology & Parasitology
DOI
Peer Reviewed
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[Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion2013
Author(s)
Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
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Journal Title
Jounal of Endodontics
Volume: 39(1)
Pages: 44-48
DOI
Peer Reviewed
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