2013 Fiscal Year Final Research Report
Analysis of Cell response by low dose irradiation
| Project/Area Number |
24792003
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
TOMITA Kazuo 鹿児島大学, 医歯(薬)学総合研究科, 助教 (60347094)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 放射線 / 遺伝子発現変化 / 酸化ストレス / ミトコンドリア / 低線量放射線影響 |
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| Research Abstract |
The cell response of low dose (0.1-100mGy) irradiation was investigated. Quantitative PCR was carried out whether specific gene expression change or not. As a result, 0.1 to 100mGy irradiation, that dose do not affect apoptosis ratio significantly, cause specific gene expression change such as p53, MnSOD in neuroblastoma cell line NB-1. Furthermore, to detect mitochondria DNA mutation, RFLP, SSCP and TaqMan based QPCR was performed. As a result, The TaqMan probe method was the easiest and most reproducible among them.
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