Identification of immunomodulatory factors of dental pulp stem cells aiming at the pulp regeneration by allogeneic transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24792164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: National Center for Geriatrics and Gerontology
• Principal Investigator: MURAKAMI Masashi 独立行政法人国立長寿医療研究センター, 再生歯科医療研究部, 研究員 (30614531)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 歯髄幹細胞 / 歯髄幹細胞培養上清 / 混合リンパ球培養反応

Outline of Final Research Achievements

The conditioned medium (CM) of dental pulp stem cells (DPSCs) was found to be the most effective in immunomodulation compared to the CM of bone marrow stem cells (BMSCs) and adipose stem cells (ADSCs). We investigated the immunomodulatory factors present in the CM of DPSCs compared to those of BMSCs and ADSCs using microarray data of pulp, BM, and adipose CD31- SP cells. The highest expressed factors in pulp CD31- SP cells compared to BM and adipose CD31- SP cells were further investigated using Real-time RT-PCR and Milliplex analysis.
MCP-1 (monocyte chemoattractant protein-1) level was higher in DPSCs CM than any other chemokine or cytokine. Moreover, MCP-1 suppressed the inflammatory response induced by IFN-γ and LPS in vitro. All these results suggested that MCP-1 was involved in the immunomodulatory effect of the CM of DPSCs.

Free Research Field

再生医療