2013 Fiscal Year Final Research Report

Keratin 17 promotes the activity of the oral squamous cell carcinoma cell: Comparison analysis with the behavior of 14-3-3 sigma

Research Project

Project/Area Number	24792185
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Surgical dentistry
Research Institution	Niigata University
Principal Investigator	MIKAMI Toshihiko 新潟大学, 医歯学総合病院, 特任助教 (90595745)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	口腔扁平上皮癌 / 上皮内癌 / keratin 17 / 14-3-3 sigma / siRNA
Research Abstract	We determined immunohistochemical profiles of 14-3-3 sigma and Keratin(K) 17 in tissue specimens of oral borderline malignancies and SCC as well as in ZK-1, an oral SCC cell line. In addition, to elucidate function of K17 in carcinoma cells, we examined cell proliferation and migration of ZK-1 cells when their K17 gene expressions were suppressed by knockdown using K17-siRNA (K17-siRNA cells). Both 14-3-3 sigma and K17 were similarly expressed in the cytoplasm of oral SCC and CIS cells but not in normal or dysplastic epithelia at tissue levels. They both were demonstrated in the cytoplasm of control ZK-1 cells, while 14-3-3 sigma was converted from the cytoplasm into the nucleus in K17-siRNA cells. The proliferation of K17-siRNA cells was significantly suppressed on day 4 after seeding. In addition, they migrated slower in scratch wound assays. The results indicate the K17 expression play an important role in promoting carcinoma cell growth in association with 14-3-3 sigma.

(1 results)