Evaluation mesenchymal stem cell conditioned medium for prevention of oral mucositis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24792199
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nagoya University
• Principal Investigator: FURUE Hiroki 名古屋大学, 医学部附属病院, 助教 (40567012)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 放射線口内炎 / 口腔癌 / 放射線治療

Research Abstract

Oral mucosits is the major side effect by radiation therapy for oral cancer. Today there is no efficient and safe therapeutic solution for oral mucositis. Our earlier studies showed that mesenchymal stem cell-conditioned medium(CM) contains many growth factors derived from stem cells, and accelerate skin wound healing. Therefore we investigated the treatment effect of CM on oral mucositis. First, rats were used for pathological assessment. Rats were treated with mouth radiation alone or in combination with CM. 5 weeks after radiation, tongue specimens were collected. There is no significant difference between radiation alone and CM. Next, for investigating the anti-inflammatory effect of the CM, some inflammatory cytokines were assayed by q-PCR or ELISA by using other animal model of inflammatory disorder. In the result, expression level of some cytokines were reduced by CM. Further studies on the mechanism of CM to protect oral mucosits by radiation therapy are needed.

Research Products

• [Journal Article] Pathological analysis of Ki-67 and CD109 expression in tongue squamous carcinoma Journal of Oral and Maxillofacial Surgery (2013)
  • Author(s): S. Hagiwara, N Yamamoto, H Furue, H Sakakura, T Shigetomi, Y Murakumo, H Hibi, M Takahashi, M Ueda
  • Journal Title: Medicine and Pathology Volume: 25(3) Pages: 276-281
  • DOI: 10.1016/j.ajoms.2012.10.002
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌患者に対する上部消化管内視鏡検査の有用性について (2014)
  • Author(s): 西川雅也, 山本憲幸, 古江浩樹, 萩原純孝, 山口聡, 石川純, 若山博隆, 日比英晴, 上田実
  • Organizer: 第32回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 札幌市 札幌コンベンションセンター
  • Year and Date: 20140123-24
• [Presentation] Exosome 構成タンパクとしてのCD109 (2013)
  • Author(s): 坂倉寛紀, 萩原純孝, 山本憲幸, 古江浩樹, 長嶺健二郎, 西川雅也, 日比英晴, 高橋雅英, 上 田 実
  • Organizer: 第58回日本口腔外科学会・学術大会
  • Place of Presentation: 福岡市 福岡国際会議場
  • Year and Date: 20131003-11
• [Presentation] 副甲状腺機能亢進症に関連し下顎骨に生じた骨形成繊維腫の一例 (2013)
  • Author(s): 山口聡, 山本憲幸, 西川雅也, 古江浩樹, 萩原純孝, 長嶺健二郎, 玉利正之, 石川純, 日比英晴, 上田実
  • Organizer: 第58回日本口腔外科学会・学術大会
  • Place of Presentation: 福岡市 福岡国際会議場
  • Year and Date: 20131003-11
• [Presentation] 関節頭付き再建プレートと遊離腓骨皮弁を用いた下顎再建 (2013)
  • Author(s): 八木俊路朗, 鳥山和宏, 高成啓介, 澤村尚, 神戸未来, 山本憲幸, 古江浩樹, 重冨俊雄, 亀井譲
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 東京都新宿区 京王プラザホテル
  • Year and Date: 20130613-14
• [Presentation] 舌に生じた巨大血管腫の一例 (2013)
  • Author(s): 古江浩樹, 山本憲幸, 西川雅也, 萩原純孝, 玉利正之, 日比英晴, 上田実
  • Organizer: 第31回日本口腔腫瘍学会
  • Place of Presentation: 東京都 秋葉原コンベンションセンター
  • Year and Date: 20130123-24
• [Presentation] 愛知県がんセンターの取り組み (2013)
  • Author(s): 古江浩樹, 西川雅也, 澤村昌嗣, 市村典久, 阿部厚, 吉岡文, 長縄弥生, 高松磨由子, 山本憲幸, 栗田賢一, 上田実, 長谷川泰久
  • Organizer: 第30回東海頭頸部腫瘍研究会
  • Place of Presentation: 名古屋市 ウインク愛知
  • Year and Date: 2013-02-02