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2013 Fiscal Year Final Research Report

Study of hyperthermic sensitization as a target of DNA repair pathways in the oral cancer cells

Research Project

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Project/Area Number 24792244
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionNara Medical University

Principal Investigator

KAJIHARA Atsuhisa  奈良県立医科大学, 医学部, 研究員 (00382317)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords臨床腫瘍学
Research Abstract

We investigated the possibility that the inhibition of homologous recombination (HR) repair or non-homologous end joining (NHEJ) repair enhances the heat sensitivity in cancer cells.There was no difference in the heat sensitivities of Ku80 defective cells and the parental cells. But, BRCA2-mutated cells were sensitive to heat as compared with the parental cells. In human tongue cancer cells, BRCA2-siRNA transfected cells were more sensitive to heat than the negative control-siRNA transfected cells. Apoptotic bodies were increased more efficiently in the BRCA2-siRNA transfected cells than the negative control-siRNA transfected cells after heat. A G2/M phase arrest was observed in the parental cells, but not in BRCA2-mutated cells after heat. DSBs were decreased at 18 h in the parental cells as compared with them at 0.5 h after heat. In contrast, they were not decreased at all in the BRCA2-mutated cells.These results suggest enhancement of heat sensitivity by depression of HR repair.

  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] The BRCA2 gene is a potential molecular target during 5-fluorouracil therapy in human oral cancer cells2014

    • Author(s)
      Nakagawa Y, Kajihara A, Takahashi A, Kondo N, Mori E, Kirita T, Ohnishi T
    • Journal Title

      Oncol Rep

      Volume: 31(5) Pages: 2001-6

    • DOI

      10.3892/or.2014.3080

    • Peer Reviewed
  • [Journal Article] Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation2012

    • Author(s)
      Nakagawa Y., Takahashi A., Kajihara A., Yamakawa N., Imai Y., Ota I., Okamoto N., Mori E., Noda T., Furusawa Y., Kirita T., Ohnishi T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 423(4) Pages: 654-60

    • DOI

      10.1016/j.bbrc.2012.06.004

    • Peer Reviewed
  • [Presentation] DNA 修復を標的とした温熱増感効果2013

    • Author(s)
      梶原淳久
    • Organizer
      日本ハイパーサーミア学会第30回大会
    • Place of Presentation
      横浜シンポジア
    • Year and Date
      20130830-31
  • [Presentation] 口腔癌細胞に対する DNA 修復を標的とした温熱増感効果についての検討2012

    • Author(s)
      梶原淳久
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20121019-21
  • [Presentation] Enhancement of heat sensitivity by depression of DSB repair2012

    • Author(s)
      Atsuhisa Kajihara
    • Organizer
      ICHO&JCTM2012
    • Place of Presentation
      Hyatt Regency Kyoto
    • Year and Date
      20120828-31
  • [Presentation] 口腔癌細胞に対する相同組換え修復を標的とした温熱増感効果についての検討2012

    • Author(s)
      梶原淳久
    • Organizer
      第66回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      20120517-18

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Published: 2015-06-25  

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