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2013 Fiscal Year Final Research Report

Dynamics and signal reception of the Wnt morphogen.

Research Project

  • PDF
Project/Area Number 24870031
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

MII Yusuke  基礎生物学研究所, 分子発生学研究部門, 助教 (70634129)

Project Period (FY) 2012-08-31 – 2014-03-31
KeywordsWnt / ヘパラン硫酸
Research Abstract

1. Based on quantitative analyses including fluorescence correlation spectroscopy (FCS) and fluorescence decay after photoconversion (FDAP), a mathematical model of extracellular behaviour of morphogens with two states (freely diffusing and bound molecules) was constructed. This model well describes accumulation of morphogen molecules, which is difficult with previous one-state models with diffusing molecules only.
2. Antibodies against Xenopus Wnt8 and Wnt11 were generated and using these antibodies, distribution of endogenous Wnt proteins were visualized. Endogenous Wnt proteins show punctate distributions similar to those of exogenous Wnt proteins. Puncta of endogenous Wnt proteins were colocalized with N-sulfo heparan sulfate (HS). It is also suggested that NDST1, which is involved in N-sulfo HS formation, is required for proper distribution of endogenous Wnt proteins.

  • Research Products

    (5 results)

All 2014 2013 2012 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] Spatial localization of noncanonical Wnt proteins during the early Xenopus embryogenesis.アフリカツメガエル初期胚における非標準経路Wnt蛋白質の空間的局在2014

    • Author(s)
      Yusuke Mii, Ritsuko Takada, Masanori Taira, Shinji Takada
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      ウィンク愛知、名古屋
    • Year and Date
      20140527-30
  • [Presentation] Heparan sulfate nanostructures regulate distribution and signal reception of secreted proteins2013

    • Author(s)
      Yusuke Mii
    • Organizer
      EMBO Workshop Morphogen Gradients 2013
    • Place of Presentation
      Lady Margaret Hall, Oxford,(イギリス)
    • Year and Date
      20130626-29
  • [Presentation] Heparan sulfate nanostructures regulate the range and signal reception of the Wnt morphogen, via endocytosis and signalosome formation2012

    • Author(s)
      Yusuke Mii
    • Organizer
      14th International Xenopus Conference
    • Place of Presentation
      Belambra Clubs, Giens Peninsula(フランス)
    • Year and Date
      20120909-13
  • [Presentation] Heparan sulfate nanostructures (HSNSs)によるWntモルフォゲンの分布およびシグナル受容制御2012

    • Author(s)
      三井優輔
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場、福岡市
    • Year and Date
      2012-12-14
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/cib2/

URL: 

Published: 2015-07-16  

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