2013 Fiscal Year Final Research Report
Identification of novel host factors responsible for the functional regulation of influenza virus ribonucleoprotein complexes
| Project/Area Number |
24890029
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | インフルエンザウイルス / 宿主因子 / リボヌクレオプロテイン / 微小管 / 細胞内輸送 |
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| Research Abstract |
The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.
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[Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K, Akimitsu N
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Journal Title
Mol. Cell
Volume: 53(3)
Pages: 393-406
Peer Reviewed
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[Journal Article] Critical role of antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity2012
Author(s)
Onomoto K, Jogi M, Yoo JS, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
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Journal Title
PLoS one
Volume: 7(8)
Pages: e43031
DOI
Peer Reviewed
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