2013 Fiscal Year Final Research Report

Bone regeneration by gene activated matrix combined with nano-size calcium phosphate particle and minicircle DNA

Research Project

Project/Area Number	24890167
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Surgical dentistry
Research Institution	Nagasaki University
Principal Investigator	MIURA Kei-ichiro 長崎大学, 医歯(薬)学総合研究科, 助教 (10634446)
Project Period (FY)	2012-08-31 – 2014-03-31
Keywords	骨再生 / Gene Activated Matrix / 口腔外科 / リン酸カルシウム / ナノサイエンス
Research Abstract	By making use of minicircle DNA, this plasmid was sucsessfully made.And we found that cell transplantaiton efficiency of this plasmid was higher than conventional plasmid. After that we prepared minicircle DNA/calcium phosphate particle/collagen composite combined with Green Fluorescent Protein gene, and assessed the material in vivo analysis. During this period, we successfully made nano-submicro calcium phosphate particle and reported the osteoconduction of the material(K Miura,et al.Applied Surface Science.2013). Furthermore, we obtained the knowledge of bone regeneration material(A Matsui,et al.Cleft Palate-Craniofacl J,2013).