2013 Fiscal Year Final Research Report

Inovation of treatment strategy for xerostomia based on hyperthermia

Research Project

Project/Area Number	24890215
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Prosthetic dentistry
Research Institution	Kyushu Dental College
Principal Investigator	MUKAIBO TARO 九州歯科大学, 歯学部, 助教 (50635117)
Project Period (FY)	2012-08-31 – 2014-03-31
Keywords	ハイパーサーミア / 口腔乾燥症
Research Abstract	The physiological and pharmacological responses of an ex vivo mouse submandibular gland were used to study fluid secretion and cell signaling in response to muscarinic stimulation at increasing temperatures. Experimental agonist carbachol (CCh) induced saliva production at 37 degrees Celsius was increased compared with that at 25 degrees Celsius. Whereas, saliva secretion induced by clinically used muscarinic agonist cevimeline at 42 degrees Celsius increased compared with that at 37 degrees Celsius. However, The calcium ionophore A23187 failed to have a significant effect on saliva production. The CCh-induced increase in intracellular Ca2+ also upregulated the initial increase and sustained the plateau phase of saliva flow. Thus, muscarinic receptor stimulation of saliva production is temperature sensitive due to an increase in intracellular Ca2+.