2012 Fiscal Year Final Research Report

Analysis of thespontaneous regeneration mechanisms in the utricle hair cells

Research Project

Project/Area Number	24890251
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Jikei University School of Medicine
Principal Investigator	UDAGAWA Tomokatsu 東京慈恵会医科大学, 医学部, 助教 (60328292)
Research Collaborator	SAIJO Hiroki 東京慈恵会医科大学, 医学部, 大学院博士課程 4 年生
Project Period (FY)	2012
Keywords	内耳 / 卵形嚢 / 有毛細胞 / 再生
Research Abstract	We found that neural crest cells spontaneously regenerate the utricle hair cells. We performed the utricle organ culture using GFP mice labeling neural crest cells. After completely lost the hair bundles, the utricles were subsequently cultured for 14 days. GFP positive cells were existed in the sensory epithelia of the cultured utricles. A part of GFP positive cells expressed Myosin VIIa, a utricle hair cell marker, in the utricle sensory epithelia. These results revealed that neural crest cells compose the utricle hair cells regenerated spontaneously.