2013 Fiscal Year Final Research Report
Analysis of a novel membrane estrogen receptor signaling and identification of its gene
| Project/Area Number |
24890286
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | エストロゲン / 細胞膜受容体 |
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| Research Abstract |
Most of estrogen actions are mediated by classic nuclear receptor, which called genomic action and regulate expression of various genes. Recently, membrane receptor mediated effects of estrogen have been identified.
In this study, I tried to analysis of a novel membrane type estrogen receptor signaling in bovine adrenal medullary cells and to cloning the receptor gene. Although, activation of already known receptors leads to increase of intracellular calcium and cAMP, estrogen did not stimulate both reactions in bovine adrenal medullary cells. These results indicated that signaling pathway of the novel receptor is completely different from the known estrogen membrane receptors. To explore the receptor gene, phage cDNA library made from mRNA of bovine adrenal medullary cells was subjected to panning with polyclonal antibody against cell membrane fraction of bovine adrenal medulla. As a result, I identified several interesting genes.
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