Development of a spectroscopic study for radiation damage induction by thermal relaxation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25241010
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Ukai Masatoshi 東京農工大学, 工学(系)研究科(研究院), 教授 (80192508)
• Co-Investigator(Kenkyū-buntansha): YOKOYA Akinari 日本原子力研究開発機構, 量子ビーム応用研究センター, 研究主幹 (10354987) ; SHIMADA Hiroyuki 東京農工大学, 大学院工学研究院, 助教 (30542112)
• Research Collaborator: FUJII Kentaro 日本原子力研究開発機構, 量子ビーム応用研究センター, 研究副主幹 ; SAITOH Yuji 日本原子力研究開発機構, 関西光量子研究所放射光科学研究センター, 研究副主幹 ; FUKUDA Yoshihiro 日本原子力研究開発機構, 関西光量子研究所放射光科学研究センター, 技術職員 ; OKUIZUMI Naoto 東京農工大学, 大学院工学府, 博士前期課程 ; SAKUMA Ichiro 東京農工大学, 大学院工学府, 博士前期課程 ; TAKEDA Yasuaki 東京農工大学, 大学院工学府, 博士前期課程 ; MIURA Ryousuke 東京農工大学, 大学院工学府, 博士前期課程 ; KASHIWADA Takumi 東京農工大学, 大学院工学府, 博士前期課程 ; SHINOZAWA Yui 東京農工大学, 大学院工学府, 博士前期課程 ; MURAI Ryuji 東京農工大学, 大学院工学府, 博士前期課程
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: DNA放射線損傷 / その場修復 / 緩和的損傷定着 / 高速電子線エネルギー損失分光法 / 液体分子線 / 時間相関分光法

Outline of Final Research Achievements

As well as the enzymatic repair for the radiation damage of DNA, a self-assembled repair is proposed to occur thermodynamically to restore the locally absorbed excess energy in a cell from radiation. Induction of radiation damage and self-assembled repair are the alternative thermal relaxation processes of bio-cell after irradiation, on which, however, much is not known. To investigate the radiation damage induction to DNA and its self-assembled repair, we have developed a new spectroscopic study to observe and bridge the initial energy deposition and the following chemical reaction under cell-mimetic condition. We thus developed synchrotron-radiation photoelectron spectroscopy and high-energy electron energy-loss spectroscopy in combination with a liquid-micro-jet sampling. For the analysis of molecular specie appearing time-dependently after irradiation, a high resolution mass spectrometry and fluorescence spectroscopy have also been developed.

Free Research Field

原子・分子物理学