Exploration of natural products and small molecues for mode-of-action study on biomembrane lipids

2016 Fiscal Year Final Research Report

• Project/Area Number: 25242073
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Osaka University
• Principal Investigator: Murata Michio 大阪大学, 理学研究科, 教授 (40183652)
• Co-Investigator(Kenkyū-buntansha): 梅川 雄一 大阪大学, 理学(系)研究科(研究院), 助教 (20587779) ; 土川 博史 大阪大学, 理学(系)研究科(研究院), 助教 (30460992) ; 松森 信明 九州大学, 理学(系)研究科(研究院), 教授 (50314357) ; 花島 慎弥 大阪大学, 理学(系)研究科(研究院), 講師 (50373353) ; 松岡 茂 大阪大学, 理学(系)研究科(研究院), 准教授 (60456184)
• Project Period (FY): 2013-05-31 – 2017-03-31
• Keywords: コレステロール / 脂質二重膜 / 脂質ラフト / 小分子プローブ / 重水素固体NMR / 表面プラズモン共鳴 / 重水素固体ＮＭＲ

Outline of Final Research Achievements

In this research, we first explored natural products that alter the signal transduction in biological membranes by binding to lipid rafts. At the same time, these lipid-specific molecular probes help to extend the area of the chemical biology to lipid membrane area. During the research period, we were able to achieve the initial goals as already published in 26 scientific papers. For example, cyclic peptides, theonellamides, which was known to bind to sterols in joint research, was revealed to interact with membrane sterol in a different way from other sterol-associating compounds like saponins. Remarkable progress of sterol recognition mechanism have been obtained by the mechanism-of-action studies on amphidinol 3 and amphotericin B.

Free Research Field

ケミカルバオロジー