Structural plasticity and functional integration of multi-functional and multi-domain proteins: From structures to systems

2016 Fiscal Year Final Research Report

• Project/Area Number: 25251019
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: Takada Shoji 京都大学, 理学研究科, 教授 (60304086)
• Co-Investigator(Kenkyū-buntansha): 検崎 博生 国立研究開発法人理化学研究所, その他部局等, 研究員 (20402464)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: タンパク質 / ｐ53 / DnaA / Ste5 / シミュレーション

Outline of Final Research Achievements

For DNA replication ignition complex made of DnaA and others, we constructed a coarse-grained model first, which is reverse-mapped to obtain atomic structure of the complex for the first time. The constructed model is made of three sub-complexes. By biochemical experiments, it was shown to be consistent with the experimental data. This study revealed near-atomic structural model of the DNA replication initiation complex.

Free Research Field

生物物理