Metagenomic and metatranscriptomic analysis of rice paddy soil

2016 Fiscal Year Final Research Report

• Project/Area Number: 25252013
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: Senoo Keishi 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)
• Co-Investigator(Kenkyū-buntansha): 大塚 重人 東京大学, 農学生命科学研究科, 准教授 (10313074) ; 磯部 一夫 東京大学, 農学生命科学研究科, 助教 (30621833)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Masahira 早稲田大学, 先進理工学研究科, 教授 (70175537) ; KUROKAWA Ken 国立遺伝学研究所, 生命情報研究センター, 教授 (20343246) ; TAKAMI Hideto (独)海洋研究開発機構, 海洋・極限環境生物領域, チームリーダー (70359165)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 水田土壌 / メタゲノム解析 / メタトランスクリプトーム解析 / メタン生成・消去 / 窒素固定 / DNRA / 脱窒

Outline of Final Research Achievements

Waterlogged paddy soil is characterized by surface oxidized and lower reduced layers, where various kind of oxidation/reduction reactions proceed. Shotgun DNA/RNA sequencing analysis (metagenomics and metatranscriptomics) of both layers soil samples revealed whole picture of microbial communities involved in methane generation and oxidation. Our analysis also revealed that most reductive nitrogen transformation (nitrogen fixation, DNRA and denitrification) gene transcripts were derived from Deltaproteobacteria, particularly the genera Anaeromyxobacter and Geobacter, previously known as iron reducers. This finding suggests the important role of these bacteria for nitrogen fertility of paddy soil.

Free Research Field

農学、農芸化学、土壌学