Molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae

2015 Fiscal Year Final Research Report

• Project/Area Number: 25252021
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAYAMA Seiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70273836)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 植物 / シグナル伝達 / 細胞間認識 / 生殖 / 自家不和合性 / アブラナ科 / ナス科

Outline of Final Research Achievements

This study aimed to reveal the molecular mechanisms of self-incompatibility in the Brassicaceae and the Solanaceae. In the Brassicaceae, SI is mediated by a "self (= same S-haplotype)" specific interaction between pollen ligand (SP11) and its stigmatic receptor (SRK). We found that calcium signaling mediated by glutamate receptor is the major downstream event of SI signaling that triggers rejection of self-pollen. In the Solanaceae, SI is mediated by the absence of "self" specific interaction between style ribonuclease (S-RNase) and pollen F-box proteins (SLFs). S-RNase exerts cytotoxicity inside self-pollen tube to inhibit its growth. We found in Petunia that 18 types of SLFs form SCF E3 ubiquitin ligase complexes and collaboratively recognize and detoxify entire suite of "non-self" S-RNases.

Free Research Field

細胞間情報学