Regulation of genome reprogramming by the mobilization of maternal mRNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 25252054
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Aoki Fugaku 東京大学, 大学院新領域創成科学研究科, 教授 (20175160)
• Project Period (FY): 2013-10-21 – 2017-03-31
• Keywords: リプログラミング / 母性mRNA / 1細胞期胚 / 卵 / 初期胚

Outline of Final Research Achievements

There are the maternal mRNA species which are not translated in immature oocytes but become being translated by elongation of their poly-A tails after the resumption of meiosis and fertilization. I hypothesized that the protein(s) regulating the reprogramming of gene expression is (are) translated from this type of maternal mRNAs. In this project, I planned to identify them and clarify their involvement in the reprogramming of gene expression.
First, we developed the system by which the maternal mRNAs elongating their poly-A tails were efficiently sorted. The selected mRNAs by using this system were subjected to RNA sequencing to determine the candidates for the factors involved in the reprogramming. The involvement of those candidates in the reprogramming has been examined by CRISPER/Cas9 system.

Free Research Field

動物育種繁殖学