Functional identification of anhydrobiosis-related genes using genome editing technology

2016 Fiscal Year Final Research Report

• Project/Area Number: 25252060
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Insect science
• Research Institution: National Agriculture and Food Research Organization (2016); National Institute of Agrobiological Sciences (2013-2015)
• Principal Investigator: Kikawada Takahiro 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 新産業開拓研究領域, 上級研究員 (60414900)
• Co-Investigator(Kenkyū-buntansha): 山本 卓 広島大学, 理学(系)研究科(研究院), 教授 (90244102)
• Co-Investigator(Renkei-kenkyūsha): CORNETTE Richard 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 新産業開拓研究領域, 主任研究員 (20376586)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 乾燥耐性 / ゲノム編集

Outline of Final Research Achievements

Our aim of this study is to reveal the gene network underlying the extreme desiccation tolerance in the desiccation tolerant cultured cells, Pv11, derived from the sleeping chironomid using the genome editing, such as CRISPR/Cas9.At first, we deciphered genome sequence of the sleeping chironomid to seek potential targets for CRISPR/Cas9, and consequently found about 2 million sites as the targets in the genome. Simultaneously, we developed a CRISPR/Cas9 applicable to Pv11 cells based on the originally isolated promoter from the cells. The optimized CRISPR/Cas9 allows us to conduct the genome-wide screening to identify the genes responsible to anhydrobiosis in Pv11 cells.

Free Research Field

昆虫分子生物学・生化学