Studies on Salmonella infection and host response based on the interaction between effector and its host target

2015 Fiscal Year Final Research Report

• Project/Area Number: 25253029
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: Yamamoto Tomoko 千葉大学, 真菌医学研究センター, 特任教授 (60110342)
• Co-Investigator(Kenkyū-buntansha): TAKAYA Akiko 千葉大学, 大学院薬学研究院, 准教授 (80334217)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: エフェクター / サルモネラ / 細菌病原性 / Caspase-8 / HO-1

Outline of Final Research Achievements

To dissect the molecular mechanism of Salmonella infection, we previously established the accurate prediction system for screening effectors on a genome-wide scale. Here, we characterized two candidates of novel effector, STM2614 and STM1239 and revealed their functions on Salmonella infection. STM2614 was injected into macrophage cytoplasm and activated caspase-8 which has two opposing functions namely as an initiator of an apoptosis and in a non-apoptotic role including induction of the pro-inflammatory response. As a molecule in macrophage targeted by STM2614, LSm8 was identified. STM1239 was injected into macrophage cells. As a molecule in macrophage targeted by STM1239, HO-1 (hem-oxygenase-1) was identified. STM1239 is capable of binding to the C-terminal transmembrane region of HO-1, suggesting the inhibition of its translocation into nucleus. It is suggested that STM1239 contributes the Salmonella infection by inhibiting the protective effect of HO-1 on its infection.

Free Research Field

細菌学