2015 Fiscal Year Final Research Report
A Comprehensive Approach Encompassing New Therapeutics for Microsatellite-instability Associated Colorectal Neoplasms
Project/Area Number |
25253080
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
Sato Toshiro 慶應義塾大学, 医学部, 准教授 (70365245)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 大腸癌 / マイクロサテライト不安定性 / オルガノイド / DNAメチル化 / 鋸歯状腺腫 / 次世代シーケンス / マイクロアレイ |
Outline of Final Research Achievements |
We generated 55 organoid lines from colorectal neoplasms, including serrated lesions and MSI colon cancers, and elaborated their biological properties. The organoids pathophysiologically recapitulated the patient tumors and exhibited unique gene expression and genetic mutation patterns. MSI colon cancers harbored substantial number of non-synonymous mutations, allowing their robust growth in hostile conditions with poor growth factors. Similar property of the MSI colon cancer organodis was observed in the xenograft models, in which the MSI colon cancer organoids displayed significantly higher tumorigenic capacity. These data suggested that the MSI colorectal cancers distinctively differ from the other sporadic colorectal cancers in molecular and biological characters, highlighting the significance of the individual therapeutics toward eradication of the MSI colorectal cancers.
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Free Research Field |
消化器外科学
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