組織工学の手法を用いた新しい分岐部人工気管の開発

2015 Fiscal Year Annual Research Report

• Project/Area Number: 25253082
• Research Institution: Kyoto University
• Principal Investigator: 中村 達雄 京都大学, 再生医科学研究所, 准教授 (70227908)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 人工気管 / コラーゲン / 気管再生 / 代用気管 / 人工臓器 / 組織工学 / 呼吸器外科

Outline of Annual Research Achievements

本研究では、胸腔内でも安全に使用し得る分岐部人工気管を開発することを目的とした。これまで開発してきた自己組織再生型人工気管に、新たなコラーゲンを用いて安全性を向上させるべく人工気管の改良を行った。気道内面に粘膜上皮再生を促すため自己組織由来幹細胞を含浸させた系も加えた。作成したＹ字型人工気管を大型実験動物の気管再建で評価し、併行して安全な再建術式も検討した。
膜様部構造を持った代用気管はメッシュ筒の全周２／３にだけステントを入れた新しいタイプの人工気管（Ｌ＝50ｍｍ）であり、気管壁の素材はマーレックスメッシュとバイクリルメッシュの二種類のものを作成した。気管内腔の虚脱を防止するため、ポリプロピレンステントをメッシュに1mm間隔で入れている。
Y字型コラーゲンとメッシュ複合体人工気管を用いてビーグル成犬（体重10-12kg）の気管分岐部を再建した。右後側法開胸で奇静脈を切断し分岐部の背方からアプローチして、左気管支、気管、右気管支の順に吻合し、腹腔より経横隔膜的に右胸腔に挙上した大網でラッピングし組織再生を促進させた。コントロールを含めて都合26頭のビーグル成犬に再建を行なった。動物実験における長期観察として、再建した気管分岐部を気管支鏡とCTで評価した。内腔保持力と気管の変形、組織再生の病理的検討を行なっており、結果をJTVSに投稿すべく準備している。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Basic experiments of bioabsorbable materials in prevention of postoperative intrapleural adhesions following thoracotomy. (2016)
  • Author(s): Hamaji, M., Burt, BM., Date, H., Nakamura, T.
  • Journal Title: Gen Thorac Cardiovasc Surg. Volume: 64 Pages: 82-86
  • DOI: 10.1007/s11748-015-0612-1.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of Tumor-associated Stroma and Its Relationship with Tumor Hypoxia Using Dynamic Contrast-enhanced CT and 18F Misonidazole PET in Murine Tumor Models. (2016)
  • Author(s): Koyasu, S., Tsuji, Y., Harada, H., Nakamoto, Y., Nobashi, T., Kimura, H., Sano, K., Koizumi, K., Hamaji, M., Togashi, K.
  • Journal Title: Radiology. Volume: 278 Pages: 734-41
  • DOI: 10.1148/radiol.2015150416.
  • Peer Reviewed
• [Journal Article] The efficacy of a novel collagen-gelatin scaffold with basic fibroblast growth factor for the treatment of vocal fold scar. (2016)
  • Author(s): Hiwatashi, N., Hirano, S., Mizuta, M., Kobayashi, T., Kawai, Y., Kanemaru, SI., Nakamura, T., Ito, J., Kawai, K., Suzuki, S.
  • Journal Title: J Tissue Eng Regen Med. Volume: in press Pages: in press
  • DOI: 10.1002/term.2060.
  • Peer Reviewed
• [Journal Article] A successful attempt to prevent postoperative adhesions between the mediastinum and a lung: a canine model. (2015)
  • Author(s): Hamaji, M., Nakamura, T.
  • Journal Title: Gen Thorac Cardiovasc Surg. Volume: 63 Pages: 483-484
  • DOI: 10.1007/s11748-015-0563-6.
  • Peer Reviewed
• [Journal Article] A rigid and bioabsorbable material for anterior chest wall reconstruction in a canine model. (2015)
  • Author(s): Hamaji, M., Kojima, F., Koyasu, S., Nobashi, T., Tsuruyama, T., Date, H., Nakamura, T.
  • Journal Title: Interact Cardiovasc Thorac Surg. Volume: 20 Pages: 322-8
  • DOI: 10.1093/icvts/ivu416.
  • Peer Reviewed / Open Access
• [Journal Article] Treatment and Prognosis of Isolated Local Relapse after Stereotactic Body Radiotherapy for Clinical Stage I Non-Small-Cell Lung Cancer: Importance of Salvage Surgery. (2015)
  • Author(s): Hamaji, M., Chen, F., Matsuo, Y., Ueki, N., Hiraoka, M., Date, H.
  • Journal Title: J Thorac Oncol. Volume: 10 Pages: 1616-24
  • DOI: 10.1097/JTO.0000000000000662.
  • Peer Reviewed
• [Journal Article] Surgery versus SABR for resectable non-small-cell lung cancer. (2015)
  • Author(s): Hamaji, M., Groth, SS., Sugarbaker, DJ., Burt, BM.
  • Journal Title: Lancet Oncol. Volume: 16 Pages: e372
  • DOI: 10.1016/S1470-2045(15)00091-1.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pleurectomy and decortication for metastatic renal cell carcinoma. (2015)
  • Author(s): Bettenhausen, A., Hamaji, M., Burt, BM., Ali, SO.
  • Journal Title: J Thorac Cardiovasc Surg. Volume: 150 Pages: e3-5
  • DOI: 10.1016/j.jtcvs.2015.05.007.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Long-Term Outcomes of Surgical and Nonsurgical Management of Stage IV Thymoma: A Population-Based Analysis of 282 Patients. (2015)
  • Author(s): Hamaji, M., Burt, BM.
  • Journal Title: Semin Thorac Cardiovasc Surg. Volume: 27 Pages: 1-3
  • DOI: 10.1053/j.semtcvs.2015.02.005.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A meta-analysis of induction therapy for advanced thymic epithelial tumors. (2015)
  • Author(s): Hamaji, M., Ali, SO., Burt, BM.
  • Journal Title: Ann Thorac Surg. Volume: 99 Pages: 1848-56
  • DOI: 10.1016/j.athoracsur.2014.12.048.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A meta-analysis of resected metachronous second non-small cell lung cancer. (2015)
  • Author(s): Hamaji, M., Ali, SO., Burt, BM.
  • Journal Title: Ann Thorac Surg. Volume: 99 Pages: 1470-8
  • DOI: 10.1016/j.athoracsur.2014.11.033.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Video-assisted thoracoscopic lobectomy versus stereotactic radiotherapy for stage I lung cance (2015)
  • Author(s): Hamaji, M., Chen, F., Matsuo, Y., Kawaguchi, A., Morita, S., Ueki, N., Sonobe, M., Nagata, Y., Hiraoka, M., Date, H.
  • Journal Title: Ann Thorac Surg. Volume: 99 Pages: 1122-9
  • DOI: 10.1016/j.athoracsur.2014.11.009.
  • Peer Reviewed
• [Journal Article] Unicentric, multifocal castleman disease of the mediastinum associated with cerebellitis. (2015)
  • Author(s): Hamaji, M., Neal, JW., Burt, BM.
  • Journal Title: Ann Thorac Surg. Volume: 99 Pages: e7-9
  • DOI: 10.1016/j.athoracsur.2014.10.028.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Autologous bone marrow mononuclear cell transplant and surgical decompression in a dog with chronic spinal cord injury. (2015)
  • Author(s): Tamura, K., Harada, Y., Kunimi, M., Takemitsu, H., Hara, Y., Nakamura, T., Tagawa, M.
  • Journal Title: Exp Clin Transplant. Volume: 13 Pages: 100-5
  • DOI: 10.6002/ect.2013.0237.
  • Peer Reviewed / Open Access
• [Journal Article] Mechanical properties of a bioabsorbable nerve guide tube for long nerve defects. (2015)
  • Author(s): Ichihara, S., Facca, S., Liverneaux, P., Inada, Y., Takigawa, T., Kaneko, K., Nakamura, T.
  • Journal Title: Chir Main. Volume: 34 Pages: 186-192
  • DOI: 10.1016/j.main.2015.05.004.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mobile locally operated detachable end-effector manipulator for endoscopic surgery. (2015)
  • Author(s): Kawai, T., Shin, M., Nishizawa, Y., Horise, Y., Nishikawa, A., Nakamura, T.
  • Journal Title: Int J Comput Assist Radiol Surg Volume: 10 Pages: 161-9
  • DOI: 10.1007/s11548-014-1062-4.
• [Journal Article] Comparison of a hydroxyapatite-coated and an anodic oxidized titanium implant for experimentally induced peri-implantitis: Macroscopic and novel radiographic evaluations in a canine model. (2015)
  • Author(s): Nakamura, H., Matsuno, T., Hashimoto, Y., Nakamura, T., Mataga, I.
  • Journal Title: Jouranal of Hard Tissue Biology Volume: 24 Pages: 347-356
  • DOI: doi.org/10.2485/jhtb.24.347
  • Open Access
• [Journal Article] Biocompatibility and Efficacy of Collagen/Gelatin Sponge Scaffold With Sustained Release of Basic Fibroblast Growth Factor on Vocal Fold Fibroblasts in 3-Dimensional Culture. (2015)
  • Author(s): Hiwatashi, N., Hirano, S., Mizuta, M., Tateya, I., Kanemaru, SI., Nakamura, T., Ito, J., Kawai, K., Suzuki, S.
  • Journal Title: Ann Otol Rhinol Laryngol. Volume: 124 Pages: 116-25
  • DOI: 10.1177/0003489414546396.
  • Peer Reviewed
• [Journal Article] Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer. (2015)
  • Author(s): Yokota, M., Kojima, M., Higuchi, Y., Nishizawa, Y., Kobayashi, A., Ito, M., Saito, N., Ochiai, A.
  • Journal Title: Cancer Sci. Volume: 106 Pages: 466-474
  • DOI: 10.1111/cas.12615.
  • Peer Reviewed
• [Journal Article] Patterns and treatment of recurrence following pulmonary resection for colorectal metastases. (2015)
  • Author(s): Yokota, M., Kobayashi, A., Nomura, S., Nishizawa, Y., Ito, M., Nagai, K., Saito, N.
  • Journal Title: World J Surg. Volume: 39 Pages: 1758-1766
  • DOI: 10.1007/s00268-015-3006-8.
  • Peer Reviewed
• [Journal Article] Effect of intratracheal administration of adipose-derived stromal cells on bloemycin-induced lung injury in a rat model. (2015)
  • Author(s): Uji, M., Nakada, A., Nakamura, T., Hirata, K.
  • Journal Title: Osaka City Med. J. Volume: 61 Pages: 81-91
  • DOI: 10.1183/13993003.
  • Peer Reviewed / Open Access
• [Presentation] 末梢神経の再生医療とin situ Tissue Engineering（生体内再生）． (2015)
  • Author(s): 中村達雄
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2015-10-09 – 2015-10-10
• [Presentation] Effect of intratracheal administration of adipose-derived stromal cells on bleomycin-induced lung injury in a rat model. (2015)
  • Author(s): Uji, M., Kureya, Y., Yamada, K., Watanabe, T., Konishi, K., Asai, K., Nakamura, T., Hirata, K.
  • Organizer: European Respiratory Society 2015 International Congress
  • Place of Presentation: Amsterdam
  • Year and Date: 2015-09-26 – 2015-09-30
  • Int'l Joint Research
• [Presentation] in situ Tissue Engineering（生体内再生）の臨床応用． (2015)
  • Author(s): 中村達雄
  • Organizer: 第16回耳鼻咽喉科再生医学研究会
  • Place of Presentation: 福島
  • Year and Date: 2015-04-28
• [Presentation] Prevention of vocal fold scarring with local application of basic fibroblast growth factor in rat model. (2015)
  • Author(s): Suzuki, R., Hirano, S., Tateya, I., Kishimoto, Y., Hiwatashi, N., Kawai, Y., Ito, J.
  • Organizer: COSM Combined Otolaryngology Spring Meeting 2015
  • Place of Presentation: U.S.A
  • Year and Date: 2015-04-22 – 2015-04-26
  • Int'l Joint Research
• [Remarks] 京都大学再生医科学研究所 臓器再建応用分野 ホームページ
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/