Development of new therapies for intractable diseases in pediatric surgery using stem cells from human exfoliated deciduous teeth

2015 Fiscal Year Final Research Report

• Project/Area Number: 25253094
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Kyushu University
• Principal Investigator: TAGUCHI Tomoaki 九州大学, 医学(系)研究科(研究院), 教授 (20197247)
• Co-Investigator(Kenkyū-buntansha): NONAKA Kazuaki 九州大学, 歯学研究院, 教授 (90128067) ; OHGA Shouichi 山口大学, 医学系研究科, 教授 (60233053) ; NAKAYAMA Koichi 佐賀大学, 医学部, 教授 (50420609) ; ODA Yoshinao 九州大学, 医学研究院, 教授 (70291515) ; IHARA Kenji 大分大学, 医学部, 教授 (80294932) ; YAMAZA Takayoshi 九州大学, 歯学研究院, 講師 (80304814) ; YAMAZA Haruyoshi 九州大学, 歯学研究院, 講師 (30336151) ; YANAGI Yusuke 九州大学, 医学研究院, 共同研究員 (30596664) ; IEIRI Satoshi 鹿児島大学, 医学部, 教授 (00363359) ; HAYASHIDA Makoto 九州大学, 医学研究院, 共同研究員 (70452761) ; NAGATA Kouji 九州大学, 大学病院, 助教 (20419568) ; YOSHIMARU Koichiro 九州大学, 医学研究院, 共同研究員 (60711190)
• Project Period (FY): 2013-10-21 – 2016-03-31
• Keywords: 乳歯幹細胞 / 難治性疾患 / 細胞移植 / 肝再生医療 / 肺成熟 / 組織工学

Outline of Final Research Achievements

We worked on the development of new therapies for 1)liver failure, 2)inherent metabolic liver disease and 3) congenital diaphragmatic hernia, using stem cells from human exfoliated deciduous teeth (SHED). The results were as follows; 1) We demonstrated anti-inflammatory, antifibrotic and liver regenerative effects of SHED and the SHED derived hepatocytes for both acute and chronic liver failure in mice. 2) We confirmed the expression of the metabolic enzymes in the SHED derived hepatocytes, which are absent in some inherent metabolic liver disease. Furtheremore, we succeeded in biofabrication of a small liver tissue in vitro using a 3D bioprinter and developed a novel transplat method of a liver tissue. 3) We demonstrated the theraputic potential of mesenchymal stem cell transplantation for pulmonary hypoplasia in a nitrofen-induced congenital diaphragmatic hernia rat model. Furthermore, we succeeded in diaphragmatic repair by a cellular patch fabricated with a 3D bioprinter.

Free Research Field

小児外科学