Analysis of genome maintenance system by the combination of proteomics and genetic approach

2015 Fiscal Year Final Research Report

• Project/Area Number: 25281021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: hirota kouji 首都大学東京, 理工学研究科, 教授 (00342840)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: DNA修復 / DNA損傷応答 / クロマチン / ユビキチン

Outline of Final Research Achievements

DNA damages caused by environmental genotoxic agents and ionizing radiations induce genome instability. Genome instability is a cause of cancer and cellular senescence, and thus understanding of the genome maintenance system is important issue. Here we defined genome maintenance system including chromatin regulation and DNA repair as ‘Genome maintenance machinery’ and studied this by the use of genetic approach and proteomics approach. To this end, we applied SILAC technique to DT40 DNA repair mutant panel. Our comprehensive approach revealed the relationship among signal pathway, repair factor and chromatin modifications. In this report, we explain about the identification of ubiquitination target proteins of RNF4 ubiquitin ligase.

Free Research Field

生物学