Functional Analysis of Anaerobic Benzene Degrading Microbial Community by Single Cell Genomics

2015 Fiscal Year Final Research Report

• Project/Area Number: 25281043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: The University of Tokyo
• Principal Investigator: Kurisu Futoshi 東京大学, 工学(系)研究科(研究院), 准教授 (30312979)
• Co-Investigator(Kenkyū-buntansha): KASUGA Ikuro 東京大学, 大学院工学系研究科, 講師 (20431794)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: バイオレメディエーション / ベンゼン / シングルセルゲノム

Outline of Final Research Achievements

Among various mechanisms of anaerobic benzene degradation, no isolates has been obtained for the degradation under methanogenic condition, and thus little is known about the degradation mechanisms. Putative benzene degrading bacterium Hasda-A, which we identified before, has been enriched by electrochemical cultivation. Single cell of Hasda-A was obtained by cell sorting and the genome sequence was analyzed. We found Benzoyl Co-A reductase gene, which is in an important pathway of anaerobic benzene degradation. Previously known initial benzene degradation metabolism, neither calboxylase gene in iron reducing condition nor gene for methylation known in nitrate reducing condition, was found. It implied the novel initial metabolism for the methanogenic enrichment culture.

Free Research Field

環境微生物工学