Development of a method for the epigenetic analysis of single cells under a micro fluidic device

2015 Fiscal Year Final Research Report

• Project/Area Number: 25286035
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nano/Microsystems
• Research Institution: The University of Tokyo
• Principal Investigator: Oana Hidehiro 東京大学, 工学(系)研究科(研究院), 准教授 (20314172)
• Co-Investigator(Renkei-kenkyūsha): WASHIZU MASAO 東京大学, 大学院工学系研究科, 教授 (10201162) ; OKEYO KENNEDY 東京大学, 大学院工学系研究科, 助教 (10634652)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ゲノム / DNA / マイクロ流体デバイス

Outline of Final Research Achievements

We developed a new methodology for analysis of individual intact chromosomes/chromatins isolated from single cells. We developed a custom-designed microfluidic device for isolation, control of the higher order structure of individual chromosome/chromatin by changing solution conditions under a fluorescence microscope. This device is also able to perform immunofluorescence staining. The method developed here has a potential application for direct investigation of the epigenetic profile of intact chromatin fibers at individual cell level.

Free Research Field

ナノバイオテクノロジー，ソフトマター物理学