Development of electrochemical detection of food poisoning bacteria based on molecular imprinting technique

2015 Fiscal Year Final Research Report

• Project/Area Number: 25288039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional solid state chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: SHIIGI Hiroshi 大阪府立大学, 工学(系)研究科(研究院), 准教授 (70335769)
• Co-Investigator(Renkei-kenkyūsha): NAGAOKA TSUTOMU 大阪府立大学, 工学研究科, 教授 (00172510) ; TOKONAMI SHIHO 大阪府立大学, 工学研究科, 准教授 (60535491) ; NISHINO TOMOAKI 東京工業大学, 大学院理学研究科, 准教授 (80372415)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分子鋳型 / バイオ分析 / 表面分析 / バイオセンサ / 大腸菌 / 細胞鋳型

Outline of Final Research Achievements

Pathogenic bacteria in food products have caused serious problems on public health. A rapid bacterial detection method has been developed through the formation of an artificial receptor without the biological function of bacteria.The artificial receptor formed on the polymer film can identify different types of E.coli based on the O-antigen. Moreover, we have succeeded in the visualization of bacteria using gold nanoparticle-labels with the molecular modification and developed a simple detection method focusing on the electrochemical properties of bacteria.

Free Research Field

分析化学