Development of conducting polymer films imprinted with microbes and their applications to microbial sensors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25288069
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: NAGAOKA TSUTOMU 大阪府立大学, 工学(系)研究科(研究院), 教授 (00172510)
• Co-Investigator(Renkei-kenkyūsha): SHIIGI HIROSHI 大阪府立大学, 工学研究科, 准教授 (70335769)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細菌 / 鋳型 / センサ / MTT / ポリピロール / ユビキノン / メナキノン

Outline of Final Research Achievements

This research project developed sensor films for real-time microbial detection using the imprinting technique that was developed by our group. The sensor films were prepared from polypyrrole film doped with a targeted microbe, such as E. coli O157. The microbe was then removed by over-oxidation of the film, which created the cavities complimentary to the template microbe. In this research project, we focused on preparing highly selective sensor films by optimizing polymerization parameters. In addition, highly sensitive electrochemical detection technique was developed using a tetrazolium salt. These efforts were integrated to realize the fabrication of real-time microbial sensors.

Free Research Field

分析化学