Engineering three dimensional tissues using electrochemistry

2015 Fiscal Year Final Research Report

• Project/Area Number: 25289291
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Yokohama National University
• Principal Investigator: Fukuda Junji 横浜国立大学, 工学(系)研究科(研究院), 准教授 (80431675)
• Co-Investigator(Kenkyū-buntansha): Wang Picao 筑波大学, 生命環境科学研究科, 教授 (80261775) ; Watanabe Masatoshi 横浜国立大学, 工学研究院, 教授 (90273383)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オリゴペプチド / 分子動力学計算 / ニードル / Hep G2

Outline of Final Research Achievements

An oligopeptide (CAAAEKEKEKEKGRGDSP) was designed using molecular dynamics calculations to electrochemically detach cells from a culture surface. Vascular endothelial cells attached on needles via the oligopeptide layer were electrochemically detached from the needles and transferred to surrounding hydrogel, resulting in endothelialized microchannels. Further, hepatoblastoma Hep G2 cells were previously encapsulated in the hydrogel. Hep G2 cells self-organized with vasculatures in following perfusion culture and formed vascularized hepatic tissues in vitro.

Free Research Field

生物工学