2015 Fiscal Year Final Research Report
Method for determination of cellar pathway structure using multidimensional proteomics
| Project/Area Number |
25290078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Keiichi 九州大学生体防御医学研究所, 教授 (80291508)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 代謝 / プロテオミクス / シグナル伝達 |
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| Outline of Final Research Achievements |
In this study, we originally developed a novel platform for targeted proteomics, iMPAQT (in vitro proteome assisted MRM for protein absolute quantification) using genome-wide recombinant protein library and applied this method to obtain quantitative structure of the pathways in biological system. We subjected more than 18000 recombinant proteins to LC-MS/MS analysis and obtained information for targeted proteomics to construct database. Using this platform, we performed absolute quantification of proteins associated with metabolism and signal-transduction pathway across various cell lines and succeeded to obtain the quantitative structure of biochemical pathways.
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| Free Research Field |
プロテオミクス
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