Value-added advance of pathogenic fungus resources by comprehensive analysis of DNA as the indicator of species diversity

2016 Fiscal Year Final Research Report

• Project/Area Number: 25290079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservation of biological resources
• Research Institution: Chiba University
• Principal Investigator: Yaguchi Takashi 千葉大学, 真菌医学研究センター, 准教授 (60361440)
• Co-Investigator(Kenkyū-buntansha): 廣瀬 大 日本大学, 薬学部, 准教授 (20513922) ; 田中 玲子 千葉大学, 真菌医学研究センター, 助教 (60143319)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 病原真菌 / 分類 / 薬剤感受性 / 次世代シーケンサー / MALDI-TOF-MS

Outline of Final Research Achievements

We analyzed the whole-genome of Aspergillus fumigatus, A. niger and their related species preserved at the Medical mycology Center, Chiba Univ., using a next-generation DNA sequencer. Among the data, we examined the relationship between antifungal-resistance gene and MICs against antifungal. We also examined the MALDI-TOF-MS for ribosome protein on A. fumigatus and their related species and were able to detect each species. The data indicated the high validity of classification on these species. The new data were added to the data-base of pathogenic fungi and opened.

Free Research Field

医真菌