The mechanism analysis of influenza RNA polymerase

2015 Fiscal Year Final Research Report

• Project/Area Number: 25291016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: Park Sam-Yong 横浜市立大学, 生命医科学研究科, 教授 (20291932)
• Co-Investigator(Kenkyū-buntansha): Sugiyama Kanako 公益財団法人神奈川科学技術アカデミー, 革新的インフルエンザウイルス創薬プロジェクト, 研究員 (20623226) ; Urano Takeshi 島根大学, 医学部, 教授 (70293701)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: インフルエンザ / RNAポリメラーゼ / 抗体

Outline of Final Research Achievements

Influenza RNA polymerase plays vital roles in the virus life cycle and, since it is much more highly conserved than other influenza virus proteins, it is of great interest as a drug target. This laboratory has created monoclonal antibodies that bind to fragments of the polymerase and block viral replication. Antibody PA11.9 binds to the PA subunit carrying the RNA polymerisation active site.
Two other antibodies were shown to bind to the RNA-binding PB2 subunit of the polymerase, including antibody PB2 3-1.6, which has notably high affinity for its target. This antibody has been patented as a research tool and diagnostic reagent (patent number 2014-068824).

Free Research Field

構造生物学