2015 Fiscal Year Final Research Report
Identificatin and characterization of bactrial genes for coexistence with hosts
| Project/Area Number |
25291021
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIRATSUCHI Akiko 金沢大学, 医薬保健研究域薬学系, 准教授 (90303297)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 細菌感染症 / 自然免疫 / ショウジョウバエ / 大腸菌 / 遺伝子発現制御 |
|---|
| Outline of Final Research Achievements |
Bacterial infection often causes serious diseases. This could be avoided when the virulence of bacteria is kept sufficiently low. We hypothesize that bacteria possess a gene(s) that creates the coexistence of hosts and invading bacteria. This study was undertaken to identify such a bacterial gene using Escherichia coli and Drosophila melanogaster as a model bacterium and host, respectively. We identified envZ-ompR, a pair of genes coding for the two-component regulatory system EnvZ-OmpR, that mitigates the virulence of E. coli in Drosophila. The enhanced expression of envZ-ompR and the phosphorylation of EnvZ were observed under infectious state. Forced expression of ompC, one of the target genes of EnvZ-OmpR, rescued the virulence phenotype of an envZ-ompR mutant. OmpC as an outer membrane protein has been known to protect bacteria from a change of osmotic pressure. The mechanisms of the induction of envZ-ompR and a mode of actions of OmpC await further investigation.
|
| Free Research Field |
免疫生化学
|
