2015 Fiscal Year Final Research Report
New Paradigm for Theory of Protein Hydration: Elucidation of Biological Self-Assembly Processes in a Unified Manner
| Project/Area Number |
25291035
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 自己組織化 / 構造形成 / フォールディング / 分子認識 / ATP駆動タンパク質 / 水和 / エントロピー / 積分方程式論 |
|---|
| Outline of Final Research Achievements |
The structures formed by the self-assembly of biomolecules are collapsed by applying high pressures (e.g., pressure denaturation of a protein, dissociation of filamentous actin (F-actin) into monomers, and destruction of amyloid fibril). The power of the formation becomes weaker at low temperatures (e.g., cold denaturation of a protein and weakened binding of myosin to F-actin). We have shown the following: These experimental facts are governed by the biomolecule-water many-body correlation component of the effect of configurational entropy of water; protein folding, various types of denaturation, and cosolvent effects on protein stability can be elucidated in a unified manner within the same theoretical framework; the diverse molecular recognitions share the same mechanism; physics of the multidrug efflux by ABC transporter is explainable; and a novel concept can be conferred upon the mechanism of functional expression of ATP-driven proteins.
|
| Free Research Field |
理論生物物理学
|
