2015 Fiscal Year Final Research Report
Stress and amino acid-responsive mechanisms of TORC1 regulation
| Project/Area Number |
25291042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Maeda Tatsuya 東京大学, 分子細胞生物学研究所, 准教授 (90280627)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | TOR / TORC1 / ラパマイシン / アミノ酸 / ストレス顆粒 / グルタミン |
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| Outline of Final Research Achievements |
We have developed a TORC1 kinase assay, by using a membrane-preserved TORC1 preparation, that reproduces for the first time, to the best of our knowledge, the amino acid-responsive TORC1 activation in vitro. The amino acid-responsiveness of the assay system was shown to be independent of the well-known Gtr-dependent nutrient-responsive mechanism and to involve the Vps34 PI3 kinase and the Pib2 protein containing a PI(3)P-binding FYVE domain.
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| Free Research Field |
分子細胞生物学
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