2015 Fiscal Year Final Research Report
Advanced use of vitamin D hydroxylases
| Project/Area Number |
25292062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANO KENJI 京都大学, 農学研究科, 教授 (10152828)
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| Co-Investigator(Renkei-kenkyūsha) |
IKUSHIRO SHINICHI 富山県立大学, 工学部, 准教授 (50244679)
YASUTAKE YOSHIAKI 産業技術総合研究所, 生物プロセス研究部門, 研究員 (20415756)
TAMURA TOMOHIRO 産業技術総合研究所, 生物プロセス研究部門, 研究部長 (30357488)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ビタミンD / シトクロムP450 / ビタミンD水酸化酵素 / ビタミンD受容体 / 高感度検出系 |
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| Outline of Final Research Achievements |
We have successfully produced the active form of vitamin D2 by a triple variant of CYP105A1. Many metabolites of natural or chemically synthesized vitamin D were also produced by bacterial or mammalian vitamin D hydroxylases to use them as authentic standards. In addition, we have developed a novel and highly sensitive system to detect serum 25-hydroxyvitamin D3.
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| Free Research Field |
生化学
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